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PIP5K1C Polyclonal antibody

PIP5K1C Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 27640-1-AP

Synonyms

KIAA0589, LCCS3, PIP5K GAMMA, PIP5K1 gamma, PIP5K1C, PIP5Kgamma, PIP5KIgamma, PtdIns(4)P 5 kinase 1 gamma

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using PIP5K1C Polyclonal antibody (27640-1-AP)

    WB analysis of A549 using 27640-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 27640-1-AP (PIP5K1C antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PIP5K1C Polyclonal antibody (27640-1-AP)

    IHC staining of human liver cancer using 27640-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27640-1-AP (PIP5K1C antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PIP5K1C Polyclonal antibody (27640-1-AP)

    IHC staining of human liver cancer using 27640-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27640-1-AP (PIP5K1C antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inA549 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27640-1-AP targets PIP5K1C in WB, IHC,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PIP5K1C fusion protein Ag26525
Full Name phosphatidylinositol-4-phosphate 5-kinase, type I, gamma
Calculated Molecular Weight 73 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberNM_001195733
Gene Symbol PIP5K1C
Gene ID (NCBI) 23396
RRIDAB_2880936
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PIP5K1C antibody 27640-1-APDownload protocol
IHC protocol for PIP5K1C antibody 27640-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

J Immunol Res

PIPKIγ Regulates CCL2 Expression in Colorectal Cancer by Activating AKT-STAT3 Signaling.

Authors - JunLi Xue
View Article
humanIHC

EBioMedicine

Type Iγ phosphatidylinositol phosphate kinase promotes tumor growth by facilitating Warburg effect in colorectal cancer.

Authors - Wei Peng
View Article