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PKM2-specific Polyclonal antibody
PKM2-specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 15822-1-AP
Validation Data Gallery
|Positive WB detected in||A549 cells, C6 cells, HeLa cells, MCF-7 cells, Recombinant protein protein, HEK-293 cells, mouse brain tissue, N/A|
|Positive IP detected in||MCF-7 cells|
|Positive IHC detected in||human lung cancer tissue, human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
15822-1-AP targets PKM2-specific in WB, IP, IHC, IF, CoIP, chIP, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||PKM pyruvate kinase, muscle|
|Calculated molecular weight||58 kDa|
|Observed molecular weight||58 kDa|
|GenBank accession number||NM_002654|
|Gene ID (NCBI)||5315|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named as PKM1/M2. The activity of the M2 isoform (but not the M1 isoform) can be inhibited by tyrosine kinase signalling in tumourcells. The primary pyruvate kinase isoform before tumour development is PK-M1; however, the primary isoform from four independent tumours is PK-M2 (PMID:18337823). The immunogen of this antibody is M2 isoform and this antibody is specific to PKM2 isoform.
|Product Specific Protocols|
|WB protocol for PKM2-specific antibody 15822-1-AP||Download protocol|
|IHC protocol for PKM2-specific antibody 15822-1-AP||Download protocol|
|IF protocol for PKM2-specific antibody 15822-1-AP||Download protocol|
|IP protocol for PKM2-specific antibody 15822-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Oxidized Vitamin C (DHA) Overcomes Resistance to EGFR-targeted Therapy of Lung Cancer through Disturbing Energy Homeostasis.
TBC1D8 Amplification Drives Tumorigenesis through Metabolism Reprogramming in Ovarian Cancer.
Front Cardiovasc Med
Biventricular Increases in Mitochondrial Fission Mediator (MiD51) and Proglycolytic Pyruvate Kinase (PKM2) Isoform in Experimental Group 2 Pulmonary Hypertension-Novel Mitochondrial Abnormalities.
Cell Commun Signal
Blockade of glycolysis-dependent contraction by oroxylin a via inhibition of lactate dehydrogenase-a in hepatic stellate cells.
J Cell Biochem
PKM2-regulated STAT3 promotes esophageal squamous cell carcinoma progression via TGF-β1-induced EMT.
Cell Death Dis
Cloxiquine, a traditional antituberculosis agent, suppresses the growth and metastasis of melanoma cells through activation of PPARγ.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Kishor (Verified Customer) (02-22-2019)
I am very much satisfied with almost all antibodies from Proteintech. This antibody is working very good at 1:1000 dilution for human cancer and primary cells.