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PLAGL1 Polyclonal antibody

PLAGL1 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 25864-1-AP

Synonyms

Lost on transformation 1, LOT 1, LOT1, PLAGL1, Tumor supressor ZAC, ZAC, ZAC1, Zinc finger protein PLAGL1

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using PLAGL1 Polyclonal antibody (25864-1-AP)

    WB analysis of MCF-7 using 25864-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25864-1-AP (PLAGL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using PLAGL1 Polyclonal antibody (25864-1-AP)

    WB analysis of mouse heart using 25864-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 25864-1-AP (PLAGL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inMCF-7 cells, mouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25864-1-AP is PLAGL1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLAGL1 fusion protein Ag23073
Full Name pleiomorphic adenoma gene-like 1
Calculated Molecular Weight 463 aa, 51 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC074814
Gene Symbol PLAGL1
Gene ID (NCBI) 5325
RRIDAB_2880274
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PLAGL1 antibody 25864-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols