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  • Featured Product
  • KD/KO Validated

FAPP2 Polyclonal antibody

FAPP2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15410-1-AP

Synonyms

FAPP 2, FAPP2, hFAPP2, PLEKHA8

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using FAPP2 Polyclonal antibody (15410-1-AP)

    WB analysis of mouse brain using 15410-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15410-1-AP (PLEKHA8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using FAPP2 Polyclonal antibody (15410-1-AP)

    WB analysis of mouse brain using 15410-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15410-1-AP (PLEKHA8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using FAPP2 Polyclonal antibody (15410-1-AP)

    IHC staining of human kidney using 15410-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15410-1-AP (PLEKHA8 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using FAPP2 Polyclonal antibody (15410-1-AP)

    IHC staining of human kidney using 15410-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15410-1-AP (PLEKHA8 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15410-1-AP targets FAPP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAPP2 fusion protein Ag7677
Full Name pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 8
Calculated Molecular Weight 58 kDa
Observed Molecular Weight 49-58 kDa, 65 kDa
GenBank Accession NumberBC002838
Gene Symbol PLEKHA8
Gene ID (NCBI) 84725
RRIDAB_2166928
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for FAPP2 antibody 15410-1-APDownload protocol
IHC protocol for FAPP2 antibody 15410-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Exp Cell Res

FAPP2 promotes tumor cell growth in human colon cancer through activation of Wnt signaling.

Authors - Jingde Chen
  • KO Validated
View Article
humanWB, IHC

Reproduction

miR-98 is involved in missed abortion by targeting GDF6 and FAPP2.

Authors - Qi Zhu
View Article
human,mouseWB

Hematol Oncol

Downregulation of FAPP2 gene induces cell autophagy and inhibits PI3K/AKT/mTOR pathway in T-cell acute lymphoblastic leukemia

Authors - Tian Yuan
  • KD Validated
View Article