PLEKHO2 Antibody

PLEKHO2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 21356-1-AP

Synonyms

PLEKHO2, PLEKHQ1, PP1628, pp9099



Tested Applications

Positive WB detected inHeLa cells, mouse thymus tissue
Positive IHC detected inhuman lung tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The 21356-1-AP immunogen is Fusion Protein PLEKHO2 protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLEKHO2 fusion protein Ag15786
Full Name pleckstrin homology domain containing, family O member 2
Calculated molecular weight 490aa,53 kDa
Observed molecular weight 53 kDa
GenBank accession number BC008744
Gene symbol PLEKHO2
Gene ID (NCBI) 80301
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Pleckstrin homology domain-containing proteins family O member 2 (PLEKHO2)’s function remains largely unknown. Genetic polymorphisms of PLEKHO2 contribute to diabetic retinopathy (DR) development. Functional prediction analysis strengthened the likelihood of participation of PLEKHO2 in the development of DR (Eric C. Han et al., 2012).

Protocols

Product Specific Protocols
WB protocol for PLEKHO2 antibody 21356-1-APDownload protocol
IHC protocol for PLEKHO2 antibody 21356-1-APDownload protocol
IF protocol for PLEKHO2 antibody 21356-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols