PLXNB1 Polyclonal antibody
PLXNB1 Polyclonal Antibody for FC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 23795-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue |
| Positive IHC detected in | human kidney tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
| FC | See 1 publications below |
Product Information
The immunogen of 23795-1-AP is PLXNB1 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PLXNB1 fusion protein Ag20719 |
| Full Name | plexin B1 |
| Calculated Molecular Weight | 2135 aa, 232 kDa |
| Observed Molecular Weight | 210 kDa |
| GenBank Accession Number | BC146793 |
| Gene Symbol | PLXNB1 |
| Gene ID (NCBI) | 5364 |
| RRID | AB_2879326 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Semaphorins, originally identified as axon guidance molecules, have also been implicated in angiogenesis, immunoregulation, and cancer (PMID: 16584533). Plexins are main receptors for semaphorins and are thought to control many of the functional effects of semaphorins. PLXNB1 is one of the three plexin-B family members. It is a high-affinity receptor for semaphorin 4D (SEMA4D). Interaction of SEMA4D and PLXNB1 has important physiologic effects in the immune and nervous systems, and is also involved in tumor progression, especially in tumor angiogenesis (PMID: 20858260).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PLXNB1 antibody 23795-1-AP | Download protocol |
| IHC protocol for PLXNB1 antibody 23795-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Immunol Res Semaphorin4D Inhibition Improves Response to Immune-Checkpoint Blockade via Attenuation of MDSC Recruitment and Function. | ||
J Transl Med SEMA4D/PlexinB1 promotes AML progression via activation of PI3K/Akt signaling. | ||
Ann Transl Med Proteomics and metabolomics of raw rhubarb and wine-processed rhubarb in the treatment of rats with intracerebral hemorrhage. | ||
J Neuroendocrinol PLXNB1 mutations in the etiology of idiopathic hypogonadotropic hypogonadism. | ||
Ann Transl Med Methylation-based epigenetic studies and gene integration analysis of preeclampsia |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Boyan (Verified Customer) (10-04-2021) | calculated size 230 kd. this antibody recognized major bands at 35, 30 kd.
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