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  • Featured Product
  • KD/KO Validated

POLR2F Polyclonal antibody

POLR2F Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15334-1-AP

Synonyms

HRBP14.4, POLR2F, POLRF, RPABC14.4, RPABC2, RPB14.4, RPB6, RPB6 homolog, RPC15

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using POLR2F Polyclonal antibody (15334-1-AP)

    WB analysis of HeLa using 15334-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15334-1-AP (POLR2F antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using POLR2F Polyclonal antibody (15334-1-AP)

    WB analysis of HeLa using 15334-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15334-1-AP (POLR2F antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using POLR2F Polyclonal antibody (15334-1-AP)

    IHC staining of human cervical cancer using 15334-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15334-1-AP (POLR2F Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using POLR2F Polyclonal antibody (15334-1-AP)

    IHC staining of human placenta using 15334-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15334-1-AP (POLR2F Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using POLR2F Polyclonal antibody (15334-1-AP)

    IHC staining of human placenta using 15334-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15334-1-AP (POLR2F Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using POLR2F Polyclonal antibody (15334-1-AP)

    IHC staining of human cervical cancer using 15334-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 15334-1-AP (POLR2F antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using POLR2F Polyclonal antibody (15334-1-AP)

    IHC staining of human cervical cancer using 15334-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 15334-1-AP (POLR2F antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using POLR2F Polyclonal antibody (15334-1-AP)

    IHC staining of human cervical cancer using 15334-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15334-1-AP (POLR2F Antibody) at dilution of 1:200 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using POLR2F Polyclonal antibody (15334-1-AP)

    IF Staining of MCF-7 using 15334-1-AP

    Immunofluorescent analysis of MCF-7 cells, using POLR2F antibody 15334-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman cervical cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15334-1-AP targets POLR2F in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POLR2F fusion protein Ag7449
Full Name polymerase (RNA) II (DNA directed) polypeptide F
Calculated Molecular Weight 14 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberBC003582
Gene Symbol POLR2F
Gene ID (NCBI) 5435
RRIDAB_10858229
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for POLR2F antibody 15334-1-APDownload protocol
IHC protocol for POLR2F antibody 15334-1-APDownload protocol
IF protocol for POLR2F antibody 15334-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Cell

Targeted protein degradation reveals RNA Pol II heterogeneity and functional diversity

Authors - Yuanjun Li
View Article
mouseWB

Cell Biosci

Replication and transcription machinery for ranaviruses: components, correlation, and functional architecture.

Authors - Fei Ke
  • KD Validated
View Article
mouseWB

Nat Commun

The transcriptional coactivator RUVBL2 regulates Pol II clustering with diverse transcription factors

Authors - Hui Wang
View Article
mouse

Genome Biol

Cross-regulome profiling of RNA polymerases highlights the regulatory role of polymerase III on mRNA transcription by maintaining local chromatin architecture

Authors - Yongpeng Jiang
View Article