POLR2J Antibody

POLR2J Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16403-1-AP

Synonyms

hRPB14, POLR2J, POLR2J1, RPB11, RPB11A, RPB11m



Tested Applications

Positive WB detected inA549 cells, HeLa cells, human brain tissue
Positive IP detected inA549 cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16403-1-AP targets POLR2J in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POLR2J fusion protein Ag9520
Full Name polymerase (RNA) II (DNA directed) polypeptide J, 13.3kDa
Calculated molecular weight 115aa,13 kDa
Observed molecular weight 13 kDa
GenBank accession number BC024165
Gene symbol POLR2J
Gene ID (NCBI) 5439
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for POLR2J antibody 16403-1-APDownload protocol
IHC protocol for POLR2J antibody 16403-1-APDownload protocol
IF protocol for POLR2J antibody 16403-1-APDownload protocol
IP protocol for POLR2J antibody 16403-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols