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PPP2R4 Polyclonal antibody

Name: PPP2R4 Polyclonal antibody
Brand: Proteintech
SKU: 10321-1-AP

PPP2R4 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10321-1-AP

Synonyms

PP2A, PP2A, subunit B, PR53 isoform, PPP2R4, PR53, PTPA

Validation Data Gallery

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AllWBIHC

WB analysis of HEK-293 using 10321-1-AP
WB result of PP2A antibody (10321-1-AP, 1:600) with si-control and si-PP2A transfected HEK293 cells.
WB analysis using 10321-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10321-1-AP (PPP2R4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of mouse testis using 10321-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10321-1-AP (PPP2R4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 10321-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10321-1-AP (PPP2R4 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 10321-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10321-1-AP (PPP2R4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 10321-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10321-1-AP (PPP2R4 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 10321-1-AP

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Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IHC detected in	mouse testis tissue, mouse kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 7 publications below

Product Information

10321-1-AP targets PPP2R4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PPP2R4 fusion protein Ag0392
Full Name	protein phosphatase 2A activator, regulatory subunit 4
Calculated Molecular Weight	37 kDa
Observed Molecular Weight	37 kDa
GenBank Accession Number	BC002545
Gene Symbol	PPP2R4
Gene ID (NCBI)	5524
RRID	AB_2284352
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PPP2R4 antibody 10321-1-AP	Download protocol
IHC protocol for PPP2R4 antibody 10321-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nucleic Acids Res KDM6B promotes PARthanatos via suppression of O6-methylguanine DNA methyltransferase repair and sustained checkpoint response. Authors - Mingming Yang View Article
human	WB	EBioMedicine Cancer-associated Fibroblasts Promote Irradiated Cancer Cell Recovery Through Autophagy. Authors - Yongbin Wang View Article
human		J Cell Sci The role of specific PP2A complexes in the dephosphorylation of γ-H2AX. Authors - Liping Chen View Article
human	WB	Mol Cancer Ther Peptide PDHPS1 inhibits ovarian cancer growth through disrupting YAP signaling. Authors - Xinxing Pan View Article
human	WB	Front Immunol CBP Bromodomain Inhibition Rescues Mice From Lethal Sepsis Through Blocking HMGB1-Mediated Inflammatory Responses. Authors - Xiaowen Bi View Article
human	WB	Life Sci PP2A alleviates oxidized LDL-induced endothelial dysfunction by regulating LOX-1/ROS/MAPK axis. Authors - Kaicheng Xu View Article

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Research Area

PPP2R4 Polyclonal antibody

PPP2R4 Polyclonal Antibody for IHC, WB, ELISA

Validation Data Gallery

WB analysis of HEK-293 using 10321-1-AP

WB analysis using 10321-1-AP

IHC staining of mouse testis using 10321-1-AP

IHC staining of mouse testis using 10321-1-AP

IHC staining of mouse kidney using 10321-1-AP

IHC staining of mouse kidney using 10321-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

KDM6B promotes PARthanatos via suppression of O6-methylguanine DNA methyltransferase repair and sustained checkpoint response.

Cancer-associated Fibroblasts Promote Irradiated Cancer Cell Recovery Through Autophagy.

The role of specific PP2A complexes in the dephosphorylation of γ-H2AX.

Peptide PDHPS1 inhibits ovarian cancer growth through disrupting YAP signaling.

CBP Bromodomain Inhibition Rescues Mice From Lethal Sepsis Through Blocking HMGB1-Mediated Inflammatory Responses.

PP2A alleviates oxidized LDL-induced endothelial dysfunction by regulating LOX-1/ROS/MAPK axis.