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PPP2R4 Polyclonal antibody

PPP2R4 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 10321-1-AP

Synonyms

PP2A, PP2A, subunit B, PR53 isoform, PPP2R4, PR53, PTPA



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells, Jurkat cells, HepG2 cells
Positive IHC detected inmouse testis tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 4 publications below

Product Information

10321-1-AP targets PPP2R4 in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPP2R4 fusion protein Ag0392
Full Name protein phosphatase 2A activator, regulatory subunit 4
Calculated molecular weight 37 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC002545
Gene symbol PPP2R4
Gene ID (NCBI) 5524
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PPP2R4 antibody 10321-1-APDownload protocol
IHC protocol for PPP2R4 antibody 10321-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EBioMedicine

Cancer-associated Fibroblasts Promote Irradiated Cancer Cell Recovery Through Autophagy.

Authors - Yongbin Wang
ratWB

Int J Biol Macromol

Polysaccharides from Pleurotus ostreatus alleviate cognitive impairment in a rat model of Alzheimer's disease.

Authors - Yan Zhang
humanWB

Life Sci

PP2A alleviates oxidized LDL-induced endothelial dysfunction by regulating LOX-1/ROS/MAPK axis.

Authors - Kaicheng Xu
humanWB

Front Immunol

CBP Bromodomain Inhibition Rescues Mice From Lethal Sepsis Through Blocking HMGB1-Mediated Inflammatory Responses.

Authors - Xiaowen Bi
human

J Cell Sci

The role of specific PP2A complexes in the dephosphorylation of γ-H2AX.

Authors - Liping Chen