PPP1R9B Polyclonal antibody

PPP1R9B Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 55129-1-AP

Synonyms

Neurabin 2, Neurabin II, PPP1R6, PPP1R9, PPP1R9B, SPINO, Spinophilin, Spn



Tested Applications

Positive WB detected inmouse brain tissue, mouse spleen tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

55129-1-AP targets PPP1R9B in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name protein phosphatase 1, regulatory (inhibitor) subunit 9B
Calculated molecular weight 89 kDa
Observed molecular weight 120-130 kDa
GenBank accession numberNM_032595
Gene symbol PPP1R9B
Gene ID (NCBI) 84687
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

PPP1R9B, also named as PPP1R6, Neurabin-II and Spinophilin, seems to act as a scaffold protein in multiple signaling pathways. It modulates excitatory synaptic transmission and dendritic spine morphology. PPP1R9B binds to actin filaments (F-actin) and shows cross-linking activity. It may play an important role in linking the actin cytoskeleton to the plasma membrane at the synaptic junction. PPP1R9B plays a role in regulation of G-protein coupled receptor signaling, including D2Rs and alpha-adrenergic receptors. PPP1R9B probably regulates p70 S6 kinase activity by forming a complex with TIAM. The antibody is specific to PPP1R9B. Observed MW of PPP1R9B is120 kDa(PMID: 28941770).

Protocols

Product Specific Protocols
WB protocol for PPP1R9B antibody 55129-1-APDownload protocol
IHC protocol for PPP1R9B antibody 55129-1-APDownload protocol
IP protocol for PPP1R9B antibody 55129-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Toxicology

Changes in memory and synaptic plasticity induced in male rats after maternal exposure to Bisphenol A.

Authors - Chong Wang