Tested Applications
| Positive WB detected in | mouse lung tissue, U2OS cells |
| Positive IP detected in | NIH/3T3 cells |
| Positive IHC detected in | rat kidney tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12554-2-AP targets PPP2CB in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3233 Product name: Recombinant human PPP2CB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-309 aa of BC012022 Sequence: MDDKAFTKELDQWVEQLNECKQLNENQVRTLCEKAKEILTKESNVQEVRCPVTVCGDVHGQFHDLMELFRIGGKSPDTNYLFMGDYVDRGYYSVETVTLLVALKVRYPERITILRGNHESRQITQVYGFYDECLRKYGNANVWKYFTDLFDYLPLTALVDGQIFCLHGGLSPSIDTLDHIRALDRLQEVPHEGPMCDLLWSDPDDRGGWGISPRGAGYTFGQDISETFNHANGLTLVSRAHQLVMEGYNWCHDRNVVTIFSAPNYCYRCGNQAAIMELDDTLKYSFLQFDPAPRRGEPHVTRRTPDYFL Predict reactive species |
| Full Name | protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform |
| Calculated Molecular Weight | 309 aa, 36 kDa |
| Observed Molecular Weight | 36 kDa |
| GenBank Accession Number | BC012022 |
| Gene Symbol | PPP2CB |
| Gene ID (NCBI) | 5516 |
| RRID | AB_2169651 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P62714 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PPP2CB antibody 12554-2-AP | Download protocol |
| IP protocol for PPP2CB antibody 12554-2-AP | Download protocol |
| WB protocol for PPP2CB antibody 12554-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PPP2CB Polyclonal antibody (Cat# 12554-2-AP) has 4 citations published in Cell Mol Life Sci, Communications Biology, Frontiers in Cell and Developmental Biology, and Cell Signaling. The research spans hepatotoxicity and AMPK signaling, sleep homeostasis regulation, vascular endothelial NO bioavailability, and m6A RNA modification in acute myeloid leukemia. Applications cited include WB and CoIP in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Cell Mol Life Sci GADD45α alleviates acetaminophen-induced hepatotoxicity by promoting AMPK activation.
| ||
Front Cell Dev Biol BRG1 Activates PR65A Transcription to Regulate NO Bioavailability in Vascular Endothelial Cells. | ||
Cell Signal METTL14-mediated N6-methyladenosine modification of TCP1 mRNA promotes acute myeloid leukemia progression |
