PPP2R5E Polyclonal antibody

PPP2R5E Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 23885-1-AP

Synonyms

PPP2R5E



Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 23885-1-AP is PPP2R5E Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPP2R5E fusion protein Ag20891
Full Name protein phosphatase 2, regulatory subunit B', epsilon isoform
Calculated molecular weight 467 aa, 55 kDa
Observed molecular weight 51 kDa
GenBank accession numberBC092477
Gene symbol PPP2R5E
Gene ID (NCBI) 5529
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PPP2R5E antibody 23885-1-APDownload protocol
IHC protocol for PPP2R5E antibody 23885-1-APDownload protocol
IF protocol for PPP2R5E antibody 23885-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols