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PPP2R5E Polyclonal antibody

PPP2R5E Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 23885-1-AP

Synonyms

PPP2R5E

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PPP2R5E Polyclonal antibody (23885-1-AP)

    WB analysis of HeLa using 23885-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 23885-1-AP (PPP2R5E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using PPP2R5E Polyclonal antibody (23885-1-AP)

    IHC staining of human cervical cancer using 23885-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 23885-1-AP (PPP2R5E Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using PPP2R5E Polyclonal antibody (23885-1-AP)

    IHC staining of human cervical cancer using 23885-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 23885-1-AP (PPP2R5E Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PPP2R5E Polyclonal antibody (23885-1-AP)

    IF Staining of HeLa using 23885-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 23885-1-AP (PPP2R5E antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 23885-1-AP is PPP2R5E Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPP2R5E fusion protein Ag20891
Full Name protein phosphatase 2, regulatory subunit B', epsilon isoform
Calculated Molecular Weight 467 aa, 55 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC092477
Gene Symbol PPP2R5E
Gene ID (NCBI) 5529
RRIDAB_2879350
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PPP2R5E antibody 23885-1-APDownload protocol
IHC protocol for PPP2R5E antibody 23885-1-APDownload protocol
IF protocol for PPP2R5E antibody 23885-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols