Tested Applications
| Positive WB detected in | HEK-293 cells, mouse brain tissue, HeLa cells, MCF-7 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human prostate cancer tissue, human testis tissue, human kidney tissue, mouse stomach tissue, human breast cancer tissue, human placenta tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
| Positive FC (Intra) detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 ul suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15395-1-AP targets PRMT6 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7592 Product name: Recombinant human PRMT6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-316 aa of BC002729 Sequence: MIADRVRTDAYRLGILRNWAALRGKTVLDVGAGTGILSIFCAQAGARRVYAVEASAIWQQAREVVRFNGLEDRVHVLPGPVETVELPEQVDAIVSEWMGYGLLHESMLSSVLHARTKWLKEGGLLLPASAELFIAPISDQMLEWRLGFWSQVKQHYGVDMSCLEGFATRCLMGHSEIVVQGLSGEDVLARPQRFAQLELSRAGLEQELEAGVGGRFRCSCYGSAPMHGFAIWFQVTFPGGESEKPLVLSTSPFHPATHWKQALLYLNEPVQVEQDTDVSGEITLLPSRDNPRRLRVLLRYKVGDQEEKTKDFAMED Predict reactive species |
| Full Name | protein arginine methyltransferase 6 |
| Calculated Molecular Weight | 42 kDa |
| Observed Molecular Weight | 42 kDa |
| GenBank Accession Number | BC002729 |
| Gene Symbol | PRMT6 |
| Gene ID (NCBI) | 55170 |
| RRID | AB_2237723 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96LA8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PRMT6 belongs to the protein arginine methyltransferase (PRMT) family that catalyzes the mono- and dimethylation of arginine residues in a variety of proteins. PRMT6 is a nuclear protein and is predominantly expressed in testis and kidney. Western blot analysis using this antibody detected a major band around 42 kDa in HeLa cells.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for PRMT6 antibody 15395-1-AP | Download protocol |
| IF protocol for PRMT6 antibody 15395-1-AP | Download protocol |
| IHC protocol for PRMT6 antibody 15395-1-AP | Download protocol |
| IP protocol for PRMT6 antibody 15395-1-AP | Download protocol |
| WB protocol for PRMT6 antibody 15395-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PRMT6 Polyclonal antibody (Cat# 15395-1-AP) has 25 citations. These appear in journals including Nature Communications, Biomaterials, Cell Death & Differentiation, Journal for ImmunoTherapy of Cancer, Cell Reports, EMBO Reports, PLoS Biology, PLoS Pathogens, and Science Advances. Research fields span epigenetics and arginine methylation, cancer (breast, colorectal, endometrial, melanoma, lung, ovarian), neurodegeneration (Huntington's, Alzheimer's, spinobulbar muscular atrophy), diabetic complications, ferroptosis, inflammation, metabolism, and stem cell biology.
| Species | Application | Title |
|---|---|---|
Nat Commun LSD1/PRMT6-targeting gene therapy to attenuate androgen receptor toxic gain-of-function ameliorates spinobulbar muscular atrophy phenotypes in flies and mice | ||
Cell Death Differ The PRMT6/STAT1/ACSL1 axis promotes ferroptosis in diabetic nephropathy | ||
Cell Death Differ Targeting the USP7-PRMT6 epigenetic axis overcomes chemoresistance in breast cancer by coordinating H3R2me2a deposition and RNF168 methylation for DNA repair and ferroptosis blockade. | ||
Biomaterials A biomimetic arsenic-based nanozyme enhances dual epigenetic regulation to improve the efficacy of immunotherapy for acute myeloid leukaemia. | ||
J Immunother Cancer Protein arginine methyltransferase 6 enhances immune checkpoint blockade efficacy via the STING pathway in MMR-proficient colorectal cancer | ||
J Neuroinflammation PRMT6 inhibition or deficiency attenuates diabetic neuropathic pain in male mice and is associated with reduced spinal neuroinflammation, microgliosis, and altered p53-p21 signaling. |
