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PROC Polyclonal antibody

PROC Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25382-1-AP

Synonyms

Anticoagulant protein C, Autoprothrombin IIA, Blood coagulation factor XIV, PC, PROC, PROC1, Vitamin K dependent protein C

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using PROC Polyclonal antibody (25382-1-AP)

    WB analysis of mouse liver using 25382-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 25382-1-AP (PROC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using PROC Polyclonal antibody (25382-1-AP)

    IHC staining of human liver using 25382-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25382-1-AP (PROC Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using PROC Polyclonal antibody (25382-1-AP)

    IHC staining of human liver using 25382-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25382-1-AP (PROC Antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inmouse liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

The immunogen of 25382-1-AP is PROC Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PROC fusion protein Ag11328
Full Name protein C (inactivator of coagulation factors Va and VIIIa)
Calculated Molecular Weight 461 aa, 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC034377
Gene Symbol PROC
Gene ID (NCBI) 5624
RRIDAB_2880052
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PROC antibody 25382-1-APDownload protocol
IHC protocol for PROC antibody 25382-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Front Cell Dev Biol

The predictive effect of immune therapy and chemotherapy under T cell-related gene prognostic index for Gastric cancer

Authors - Jingyao Chen
View Article