• Featured Product
  • KD/KO Validated

PTK7 Polyclonal antibody

PTK7 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 17799-1-AP

Synonyms

CCK 4, CCK4, Colon carcinoma kinase 4, Protein tyrosine kinase 7, PTK7, PTK7 protein tyrosine kinase 7, Tyrosine protein kinase like 7



Tested Applications

Positive WB detected inPC-12 cells, Jurkat cells, A431 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 17799-1-AP is PTK7 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTK7 fusion protein Ag12004
Full Name PTK7 protein tyrosine kinase 7
Calculated molecular weight 1070 aa, 118 kDa
Observed molecular weight 140-150 kDa
GenBank accession numberBC071557
Gene symbol PTK7
Gene ID (NCBI) 5754
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PTK7 antibody 17799-1-APDownload protocol
IHC protocol for PTK7 antibody 17799-1-APDownload protocol
IP protocol for PTK7 antibody 17799-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Med Sci Monit

Bioinformatics Analysis Identifies Protein Tyrosine Kinase 7 (PTK7) as a Potential Prognostic and Therapeutic Biomarker in Stages I to IV Hepatocellular Carcinoma.

Authors - Ren-Chao Zou
mouseWB

Life Sci

Identification of metastasis-related genes by genomic and transcriptomic studies in murine melanoma.

Authors - Onat Kadioglu
humanIHC

Mod Pathol

USCAP 2018 Abstracts: Techniques (2229-2304).

Authors - W. D. Mojica
humanWB,IHC,IF

Cell Death Dis

Periostin secreted by cancer-associated fibroblasts promotes cancer stemness in head and neck cancer by activating protein tyrosine kinase 7.

Authors - Binbin Yu
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Gabriel (Verified Customer) (01-15-2019)

  • Applications: Immunoprecipitation,
  • Cell Tissue Type: MCF10A
PTK7 Antibody Immunoprecipitation, validation ( dilution) in MCF10A (Cat no:17799-1-AP)