PTP4A1/PRL1 Polyclonal antibody

PTP4A1/PRL1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 11508-1-AP

Synonyms

HH72, PRL 1, PRL1, PTP(CAAX1), PTP(CAAXI), PTP4A1, PTPCAAX1



Tested Applications

Positive WB detected inHEK-293 cells, MCF-7 cells, HepG2 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 3 publications below

Product Information

11508-1-AP targets PTP4A1/PRL1 in WB, IHC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTP4A1/PRL1 fusion protein Ag2067
Full Name protein tyrosine phosphatase type IVA, member 1
Calculated molecular weight 20 kDa
Observed molecular weight 18-20 kDa
GenBank accession numberBC023975
Gene symbol PTP4A1
Gene ID (NCBI) 7803
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PTP4A1(protein tyrosine phosphatase type IVA, member 1), also named as PRL1, HH72, PRL-1, PTP(CAAX1), PTPCAAX1, DKFZp779M0721, belongs to the protein-tyrosine phosphatase family, which play an important role in maintaining appropriate tyrosine phosphorylation levels in the cell during development and tissue regeneration (PMID:19341304). PTP4A1 is a PTPase and that it is highly expressed in growing hepatic cells and some tumor cell lines and is located primarily in the cell nucleus, and its overexpression modulates the growth of cells (PMID:8196618). PTP4A1 can exist as dimer, trimer and higher oligomers in vivo (PMID: 15571731, 18224294).

Protocols

Product Specific Protocols
WB protocol for PTP4A1/PRL1 antibody 11508-1-APDownload protocol
IHC protocol for PTP4A1/PRL1 antibody 11508-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncol Lett

miR-339-3p inhibits proliferation and metastasis of colorectal cancer.

Authors - Chang Zhou
humanWB

PLoS One

MiR-339-5p regulates the growth, colony formation and metastasis of colorectal cancer cells by targeting PRL-1.

Authors - Zhou Chang C
humanWB

Oncotarget

Oncogenic ALK regulates EMT in non-small cell lung carcinoma through repression of the epithelial splicing regulatory protein 1.

Authors - Claudia Voena