|Positive WB detected in||mouse pancreas tissue, rat pancreas tissue|
|Positive IHC detected in||human pancreas cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
11493-1-AP targets PTPMT1 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PTPMT1 fusion protein Ag2063|
|Full Name||protein tyrosine phosphatase, mitochondrial 1|
|Calculated molecular weight||201 aa, 23 kDa|
|Observed molecular weight||21-23 kDa|
|GenBank accession number||BC020242|
|Gene ID (NCBI)||114971|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Protein Tyrosine Phosphatase localized to the Mitochondrial 1 (PTPMT1) is a highly conserved, dual-specific phosphatase that resides on the matrix-facing side of the inner mitochondrial membrane (IMM). PTPMT1 is a mitochondrial phosphatase and a vital regulator of mitochondrial activities. Current work points to roles for PTPMT1 in the regulation of mitochondrial respiration, dynamics, and ultrastructure through the modulation of pyruvate importation, the tuning of TCA cycle activity, and the synthesis of cardiolipin. PTPMT1 is highly expressed in pancreatic b cells, whose mitochondria have the important function of coupling glucose metabolism to the secretion of insulin. (PMID: 16337125, PMID: 16061174, PMID: 33335799)
Genome-wide CRISPR-Cas9 knockout library screening identified PTPMT1 in cardiolipin synthesis is crucial to survival in hypoxia in liver cancer.
Mol Cell Biol
A critical role of mitochondrial phosphatase Ptpmt1 in embryogenesis reveals a mitochondrial metabolic stress-induced differentiation checkpoint in embryonic stem cells.
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Tongbin (Verified Customer) (08-25-2020)
This antibody detects PTPMT1 in mouse heart samples.