IA-2/PTPRN Monoclonal antibody
IA-2/PTPRN Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG3
Reactivity
human
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
1D3F5
Cat no : 66045-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human brain tissue |
| Positive IHC detected in | human pancreas cancer tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66045-1-Ig targets IA-2/PTPRN in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG3 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | IA-2/PTPRN fusion protein Ag17909 |
| Full Name | protein tyrosine phosphatase, receptor type, N |
| Calculated Molecular Weight | 105 kDa |
| Observed Molecular Weight | 60-65 kDa, 106-115 kDa |
| GenBank Accession Number | BC007713 |
| Gene Symbol | PTPRN |
| Gene ID (NCBI) | 5798 |
| RRID | AB_11064603 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IA-2/PTPRN is a member of the protein tyrosine phosphatase (PTP) family, which contains a transmembrane region, an intracellular PTP-like domain and an extracellular N-terminus. Experiments found that IA-2/PTPRN localizes to secretory granules and is exclusively expressed in neuroendocrine cells (including pancreatic islets cell). IA-2/PTPRNwas found to be a major autoantigen in insulin-dependent diabetes mellitus and the detection of autoantibodies against IA-2/PTPRN is commonly used as a diabetes diagnosis marker.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IA-2/PTPRN antibody 66045-1-Ig | Download protocol |
| IHC protocol for IA-2/PTPRN antibody 66045-1-Ig | Download protocol |
| IF protocol for IA-2/PTPRN antibody 66045-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
