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- KD/KO Validated
PURB Polyclonal antibody
PURB Polyclonal Antibody for IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, RIP, IP, IF, ChIP, ELISA
Conjugate
Unconjugated
Cat no : 18128-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | C2C12 cells, mouse liver tissue, HeLa cells |
| Positive IP detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 7 publications below |
| IF | See 2 publications below |
| ChIP | See 2 publications below |
| RIP | See 2 publications below |
Product Information
18128-1-AP targets PURB in WB, RIP, IP, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PURB fusion protein Ag12705 |
| Full Name | purine-rich element binding protein B |
| Calculated Molecular Weight | 312 aa, 33 kDa |
| Observed Molecular Weight | 33-40 kDa |
| GenBank Accession Number | BC101735 |
| Gene Symbol | PURB |
| Gene ID (NCBI) | 5814 |
| RRID | AB_2878504 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PURB antibody 18128-1-AP | Download protocol |
| IP protocol for PURB antibody 18128-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun MaTAR25 lncRNA regulates the Tensin1 gene to impact breast cancer progression. | ||
Int J Mol Sci Circular Intronic RNA circTTN Inhibits Host Gene Transcription and Myogenesis by Recruiting PURB Proteins to form Heterotypic Complexes | ||
J Cell Physiol PURB is a positive regulator of amino acid-induced milk synthesis in bovine mammary epithelial cells. | ||
J Biol Chem A novel Cbx1, PurB, and Sp3 complex mediates long-term silencing of tissue- and lineage-specific genes. | ||
