|Positive WB detected in||HeLa cells, HepG2 cells, mouse liver, rat liver|
|Positive IP detected in||mouse liver tissue|
|Positive IHC detected in||human kidney tissue, human lung tissue, human ovary tissue, human heart tissue, human testis tissue, human skin tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
15851-1-AP targets PYGL in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, squirrel|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PYGL fusion protein Ag8664|
|Full Name||phosphorylase, glycogen, liver|
|Calculated molecular weight||846 aa, 97 kDa|
|Observed molecular weight||97 kDa|
|GenBank accession number||BC009895|
|Gene ID (NCBI)||5836|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
|Product Specific Protocols|
|WB protocol for PYGL antibody 15851-1-AP||Download protocol|
|IHC protocol for PYGL antibody 15851-1-AP||Download protocol|
|IF protocol for PYGL antibody 15851-1-AP||Download protocol|
|IP protocol for PYGL antibody 15851-1-AP||Download protocol|
|Click here to view our Standard Protocols|
J Cell Physiol
Novel findings on ultrastructural protection of skeletal muscle fibers during hibernation of Daurian ground squirrels: Mitochondria, nuclei, cytoskeleton, glycogen.
Chronic liver disease and impaired hepatic glycogen metabolism in argininosuccinate lyase deficiency.
Loss of protein targeting to glycogen sensitizes human hepatocellular carcinoma cells towards glucose deprivation mediated oxidative stress and cell death.
Loss of Protein Targeting to Glycogen sensitizes human hepatocellular carcinoma cells towards glucose deprivation mediated oxidative stress and cell death.
Induction of protective immunity against Chlamydia muridarum intravaginal infection with a chlamydial glycogen phosphorylase.
1,2-Dichloroethane impairs glucose and lipid homeostasis in the livers of NIH Swiss mice.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Patricia (Verified Customer) (02-25-2020)
Works very well.