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PYGL Polyclonal antibody
PYGL Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 15851-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, mouse liver, rat liver |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human kidney tissue, human lung tissue, human ovary tissue, human heart tissue, human testis tissue, human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 13 publications below |
| IHC | See 1 publications below |
| IP | See 1 publications below |
Product Information
15851-1-AP targets PYGL in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, squirrel |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PYGL fusion protein Ag8664 |
| Full Name | phosphorylase, glycogen, liver |
| Calculated molecular weight | 846 aa, 97 kDa |
| Observed molecular weight | 97 kDa |
| GenBank accession number | BC009895 |
| Gene symbol | PYGL |
| Gene ID (NCBI) | 5836 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PYGL antibody 15851-1-AP | Download protocol |
| IHC protocol for PYGL antibody 15851-1-AP | Download protocol |
| IF protocol for PYGL antibody 15851-1-AP | Download protocol |
| IP protocol for PYGL antibody 15851-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cell Physiol Novel findings on ultrastructural protection of skeletal muscle fibers during hibernation of Daurian ground squirrels: Mitochondria, nuclei, cytoskeleton, glycogen. | ||
JCI Insight Chronic liver disease and impaired hepatic glycogen metabolism in argininosuccinate lyase deficiency. | ||
Biosci Rep Loss of protein targeting to glycogen sensitizes human hepatocellular carcinoma cells towards glucose deprivation mediated oxidative stress and cell death. | ||
Biosci Rep Loss of Protein Targeting to Glycogen sensitizes human hepatocellular carcinoma cells towards glucose deprivation mediated oxidative stress and cell death. | ||
PLoS One Induction of protective immunity against Chlamydia muridarum intravaginal infection with a chlamydial glycogen phosphorylase. | ||
Toxicology 1,2-Dichloroethane impairs glucose and lipid homeostasis in the livers of NIH Swiss mice. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Patricia (Verified Customer) (02-25-2020) | Works very well.
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