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PYGL Polyclonal antibody

PYGL Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15851-1-AP

Synonyms

GSD6, phosphorylase, glycogen, liver, PYGL



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, mouse liver, rat liver
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman kidney tissue, human lung tissue, human ovary tissue, human heart tissue, human testis tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

15851-1-AP targets PYGL in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, squirrel
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PYGL fusion protein Ag8664
Full Name phosphorylase, glycogen, liver
Calculated molecular weight 846 aa, 97 kDa
Observed molecular weight 97 kDa
GenBank accession numberBC009895
Gene symbol PYGL
Gene ID (NCBI) 5836
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PYGL antibody 15851-1-APDownload protocol
IHC protocol for PYGL antibody 15851-1-APDownload protocol
IF protocol for PYGL antibody 15851-1-APDownload protocol
IP protocol for PYGL antibody 15851-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
squirrelWB

J Cell Physiol

Novel findings on ultrastructural protection of skeletal muscle fibers during hibernation of Daurian ground squirrels: Mitochondria, nuclei, cytoskeleton, glycogen.

Authors - Zhe Wang
mouseWB

JCI Insight

Chronic liver disease and impaired hepatic glycogen metabolism in argininosuccinate lyase deficiency.

Authors - Lindsay C Burrage
humanWB

Biosci Rep

Loss of protein targeting to glycogen sensitizes human hepatocellular carcinoma cells towards glucose deprivation mediated oxidative stress and cell death.

Authors - Rongqiang Yang
humanWB

Biosci Rep

Loss of Protein Targeting to Glycogen sensitizes human hepatocellular carcinoma cells towards glucose deprivation mediated oxidative stress and cell death.

Authors - Rongqiang Yang
mouseWB

PLoS One

Induction of protective immunity against Chlamydia muridarum intravaginal infection with a chlamydial glycogen phosphorylase.

Authors - Li Zhihong Z
mouseWB

Toxicology

1,2-Dichloroethane impairs glucose and lipid homeostasis in the livers of NIH Swiss mice.

Authors - Ting Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Patricia (Verified Customer) (02-25-2020)

Works very well.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Ovarian cancer