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Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, mouse liver, rat liver |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human kidney tissue, mouse heart tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 34 publications below |
| IHC | See 4 publications below |
| IF | See 2 publications below |
| IP | See 1 publications below |
Product Information
15851-1-AP targets PYGL in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, squirrel |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PYGL fusion protein Ag8664 Predict reactive species |
| Full Name | phosphorylase, glycogen, liver |
| Calculated Molecular Weight | 846 aa, 97 kDa |
| Observed Molecular Weight | 97 kDa |
| GenBank Accession Number | BC009895 |
| Gene Symbol | PYGL |
| Gene ID (NCBI) | 5836 |
| RRID | AB_2175014 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P06737 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PYGL antibody 15851-1-AP | Download protocol |
| IHC protocol for PYGL antibody 15851-1-AP | Download protocol |
| IF protocol for PYGL antibody 15851-1-AP | Download protocol |
| IP protocol for PYGL antibody 15851-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nature Targeting SLC7A11 improves efferocytosis by dendritic cells and wound healing in diabetes. | ||
Cell Metab Fibroblasts Mobilize Tumor Cell Glycogen to Promote Proliferation and Metastasis. | ||
Cell Metab Cell-Intrinsic Glycogen Metabolism Supports Early Glycolytic Reprogramming Required for Dendritic Cell Immune Responses. | ||
J Clin Invest Ketohexokinase C blockade ameliorates fructose-induced metabolic dysfunction in fructose-sensitive mice. | ||
Int J Biol Sci PYGL-mediated glucose metabolism reprogramming promotes EMT phenotype and metastasis of pancreatic cancer |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Patricia (Verified Customer) (02-25-2020) | Works very well.
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