Paxillin Antibody

Paxillin Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, dog, mouse

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 22172-1-AP

Synonyms

paxillin, PXN



Tested Applications

Positive WB detected inRAW 264.7 cells
Positive IHC detected inhuman breast cancer tissue, human stomach cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMDCK cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The 22172-1-AP immunogen is Fusion Protein Paxillin protein expressed in E. coli.

Reactivity human, dog, mouse
Cited Species human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Paxillin fusion protein Ag17702
Full Name paxillin
Calculated molecular weight 591aa,65 kDa
Observed molecular weight
GenBank accession number BC136787
Gene symbol PXN
Gene ID (NCBI) 5829
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Paxillin antibody 22172-1-APDownload protocol
IHC protocol for Paxillin antibody 22172-1-APDownload protocol
IF protocol for Paxillin antibody 22172-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Signal

Activated CRH receptors inhibit autophagy by repressing conversion of LC3BI to LC3BII.

Authors - Lai Jin
humanWB,IHC

Oncogene

KIF26B, a novel oncogene, promotes proliferation and metastasis by activating the VEGF pathway in gastric cancer.

Authors - H Zhang
humanWB,IHC

Oncogene

KIF26B, a novel oncogene, promotes proliferation and metastasis by activating the VEGF pathway in gastric cancer.

Authors - H Zhang