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Phospho-DLK/MAP3K12 (Thr43) Polyclonal antibody

Phospho-DLK/MAP3K12 (Thr43) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28277-1-AP

Synonyms

DLK, Leucine zipper protein kinase, MAP3K12, MAPK upstream kinase, Mixed lineage kinase, MUK, Phospho-DLK/MAP3K12 (Thr43), ZPK, ZPKP1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-DLK/MAP3K12 (Thr43) Polyclonal antibody (28277-1-AP)

    WB analysis using 28277-1-AP

    Non-treated PC-3 and PDGF (HZ-1215) treated PC-3 cells were subjected to SDS PAGE followed by western blot with 28277-1-AP (Phospho-DLK (Thr43) antibody) at dilution of 1:600 incubated at 4℃ overnight.

Tested Applications

Positive WB detected inPDGF treated PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28277-1-AP targets Phospho-DLK/MAP3K12 (Thr43) in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name mitogen-activated protein kinase kinase kinase 12
Calculated Molecular Weight 93 kda
Observed Molecular Weight93 kDa
GenBank Accession NumberNM_001193511
Gene Symbol MAP3K12
Gene ID (NCBI) 7786
RRIDAB_2918153
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAP3K12, also named as DLK ((dual leucine zipper kinase), is a part of a non-canonical MAPK signaling pathway. It has been reported that MAP3K12 can be activated by APOE through a receptor-mediated mechanism. MAP3K12 enhances the AP-1-mediated transcription of APP via a MAP kinase signal transduction pathway composed of MAP2K7, MAPK1/ERK2 and MAPK3/ERK1. MAP3K12 is required for pancreatic beta-cell proliferation during postnatal development and stress-induced JNK signaling in neurons.(PMID: 28111074, 25341110, 32189007)

Protocols

Product Specific Protocols
WB protocol for Phospho-DLK/MAP3K12 (Thr43) antibody 28277-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols