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Phospho-ATR (Ser428) Recombinant monoclonal antibody (86330-1-RR)

Phospho-ATR (Ser428) Recombinant monoclonal antibody

Phospho-ATR (Ser428) Uni-rAb^® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Cat No. 86330-1-RR

Clone No.250988D3

Publications(2)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

ATR (Ser428), p ATR (Ser428), p-ATR, p-ATR (Ser428), p-ATR-Ser428

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

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Quantity

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Filter: AllWBIF/ICCFC (Intra)

WB analysis of HEK-293 using 86330-1-RR
Non-treated HEK-293 cells, phosphatase inhibitor treated HEK-293 cells and λ phosphatase treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 86330-1-RR (Phospho-ATR (Ser428) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (66305-1-Ig) antibody as a loading control.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HEK-293 using 86330-1-RR
Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated HEK-293 cells using Phospho-ATR (Ser428) antibody (86330-1-RR, Clone: 250988D3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of HEK-293 using 86330-1-RR
1X10^6 HEK-293 cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.13 ug Phospho-ATR (Ser428) Recombinant monoclonal antibody (86330-1-RR, Clone:250988D3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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1X10^6 HEK-293 cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.13 ug Phospho-ATR (Ser428) Recombinant monoclonal antibody (86330-1-RR, Clone:250988D3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).

Overview

Tested Applications
Product Information
Publications (2)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HEK-293 cells
Positive IF/ICC detected in	HEK-293 cells
Positive FC (Intra) detected in	HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86330-1-RR targets Phospho-ATR (Ser428) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	ataxia telangiectasia and Rad3 related
Calculated Molecular Weight	301 kDa
Observed Molecular Weight	300 kDa
GenBank Accession Number	NM_001184
Gene Symbol	ATR
Gene ID (NCBI)	545
RRID	AB_3744813
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q13535
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ataxia-telangiectasia and Rad3 related (ATR) is a serine/threonine kinase and a member of the phosphatidylinositol 3-kinase-related kinase (PIKK) family, particularly the ataxia telangiectasia mutated (ATM) subfamily. The phosphorylation site of ATR is located at Ser428 and is crucial for proper ATR function. In a series of breast cancer studies, high ATR expression and activation were significantly associated with higher tumor stage, mitotic index, pleomorphism, lymphovascular invasion, and poor survival. (PMID: 33854565)

Protocols

Product Specific Protocols
FC protocol for Phospho-ATR (Ser428) antibody 86330-1-RR	Download protocol
IF protocol for Phospho-ATR (Ser428) antibody 86330-1-RR	Download protocol
WB protocol for Phospho-ATR (Ser428) antibody 86330-1-RR	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
human	WB	Adv Sci (Weinh) Elevated TRIM25 Impairs Poly (ADP-ribose) Metabolism via PARG Degradation and Mediates Compression-Induced Intervertebral Disc Degeneration. Authors - Zhangrong Cheng View Article
human	WB	Drug Resist Updat RAD1 promotes oxaliplatin resistance in gastric cancer by reinforcing NRF2-driven antioxidant defense and DDR checkpoint signaling. Authors - Baichuan Zhou View Article