Tested Applications
| Positive WB detected in | EGF treated A431 cells |
| Positive IF/ICC detected in | EGF treated A431 cells |
| Positive FC (Intra) detected in | EGF treated A431 cells |
This antibody, also known as EGFR Tyr992, is the site where the signal peptide is removed.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
82736-11-RR targets Phospho-EGFR (Tyr1016) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) |
| Calculated Molecular Weight | 1210 aa, 134 kDa |
| Observed Molecular Weight | 170 kDa |
| GenBank Accession Number | BC094761 |
| Gene Symbol | EGFR |
| Gene ID (NCBI) | 1956 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P00533 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Epidermal Growth Factor Receptor (EGFR) is one of the receptor tyrosine kinases (RTKs), members of the ErbB/HER family which consists of ErbB1 (EGFR or HER1), ErbB2 (HER2 or Neu), ErbB3 (HER3), and ErbB4 (HER4). Phosphorylation at Tyr1016 is essential for the recruitment of downstream signaling molecules. For example, it can interact with adapter proteins like GRB2, which in turn activates the Ras-MAPK pathway, leading to cell proliferation and differentiation. (PMID: 26628682) Some scientists call EGFR Tyr1016 as EGFR Tyr992, the site where the signaling peptide is removed.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Phospho-EGFR (Tyr1016) antibody 82736-11-RR | Download protocol |
| IF protocol for Phospho-EGFR (Tyr1016) antibody 82736-11-RR | Download protocol |
| WB protocol for Phospho-EGFR (Tyr1016) antibody 82736-11-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
