Phospho-FOXO3A (Ser315) Polyclonal antibody

Phospho-FOXO3A (Ser315) Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 28755-1-AP

Synonyms

AF6q21, AF6q21 protein, DKFZp781A0677, FKHRL1, FKHRL1P2, forkhead box O3, Forkhead box protein O3, FOXO2, FOXO3, FOXO3A, FOXO3A (Ser315), FOXO3A S315, FOXO3A Ser315, Phospho-FOXO3A (Ser315), S315, Ser315



Tested Applications

Positive WB detected inCalyculin A treated PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

28755-1-AP targets Phospho-FOXO3A (Ser315) in WB,ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name forkhead box O3
Calculated molecular weight 673 aa, 71 kDa
Observed molecular weight80-90 kDa
GenBank accession numberBC021224
Gene symbol FOXO3
Gene ID (NCBI) 2309
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

In mammalian cells, FoxO factors act as potent transcriptional activators that upregulate the expression of programs of genes involved in stress resistance, cell cycle arrest, differentiation, apoptosis, autophagy, and metabolism. FOXO3 has a key role in the maintenance of adult neural and hematopoietic stem cells. It also associates with extreme longevity in humans and regulates the homeostasis of adult stem cell pools in mammals, which may contribute to longevity. Phosphorylation inhibits FOXO3 nuclear translocation that is essential for its regulation and function. The calculated molecular weight of FOXO3 is 71 kDa,but some experiments analysis using a FOXO3a specific antibody showed the expression of a 90 kDa full length FOXO3a protein (Full) and a 40 kDa processed FOXO3a product (Short). 90 kDa FOXO3a protein is a phosphorylated via activation of PI3K (phosphatidylinositol 3-kinase) and Akt after INS stimulation, and translocate from the nucleus to the cytoplasm. (PMID: 19427386). IKBKE directly activates Akt and that Akt inhibits FOXO3a by phosphorylation of Ser32, Ser253 and Ser315.

Protocols

Product Specific Protocols
WB protocol for Phospho-FOXO3A (Ser315) antibody 28755-1-APDownload protocol
Standard Protocols
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