Tested Applications
| Positive WB detected in | NIH/3T3 cells |
| Positive FC (Intra) detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
81251-2-RR targets Phospho-PERK/EIF2AK3 (Ser719) in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | eukaryotic translation initiation factor 2-alpha kinase 3 |
| Calculated Molecular Weight | 1116 aa, 125 kDa |
| Observed Molecular Weight | 140 kDa |
| GenBank Accession Number | BC126354 |
| Gene Symbol | PERK |
| Gene ID (NCBI) | 9451 |
| RRID | AB_3743376 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9NZJ5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
EIF2AK3 encodes the protein kinase RNA-like ER kinase (PERK), a key regulator of the unfolded protein response (UPR) in response to ER stress. Under ER stress conditions, activation of PERK is triggered by the dissociation of glucose-regulated protein (GRP) 78 (also known as BiP) from its luminal domain, followed by oligomerization and autophosphorylation. Phosphorylated PERK subsequently phosphorylates eukaryotic translation initiation factor 2 alpha (eif2α), to attenuate global protein translation and reduce incoming ER protein load via upregulated ER chaperone expression. (PMID: 35922637, PMID: 32029570)
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Phospho-PERK/EIF2AK3 (Ser719) antibody 81251-2-RR | Download protocol |
| WB protocol for Phospho-PERK/EIF2AK3 (Ser719) antibody 81251-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Phospho-PERK/EIF2AK3 (Ser719) Recombinant monoclonal antibody (243165A4), catalog number 81251-2-RR, has 1 total citation. The citation appears in Cell Stress Chaperones (Impact Factor 4.1), published in 2026. The associated research field is cancer biology, specifically investigating how inhibition of α-1,3-mannosyltransferase sensitizes head and neck squamous cell carcinomas to cetuximab via endoplasmic reticulum stress signaling. The antibody was used for Western blot analysis in human samples.
| Species | Application | Title |
|---|---|---|
Cell Stress Chaperones Inhibition of α-1,3-mannosyltransferase sensitizes head and neck squamous cell carcinomas to cetuximab via endoplasmic reticulum stress. |
