Tested Applications
| Positive WB detected in | PDGF treated HEK-293 cells, PDGF treated C6 cells, PDGF treated NIH/3T3 cells |
| Positive IF/ICC detected in | 100 nM Calyculin A (30 minutes) treated HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
80130-2-RR targets Phospho-S6 Ribosomal protein (Ser235/236) in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | ribosomal protein S6 |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | NM_001010 |
| Gene Symbol | RPS6 |
| Gene ID (NCBI) | 6194 |
| RRID | AB_3670467 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P62753 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The ribosomal protein S6 (rpS6) is a component of the small 40S ribosomal subunit implicated in mRNA decoding. rpS6 is phosphorylated at multiple sites, comprised between Ser235 and Ser247, by the p70 rpS6 kinase (S6K) 1, which is a major downstream effector of the mammalian target of rapamycin complex 1 (mTORC1). Phosphorylation of rpS6 at the dual site Ser235/236 occurs also independently of mTORC1, via the p90 ribosomal S6 kinases (RSK), which are activated by the extracellular signal-regulated kinases (ERK). (PMID: 21814187)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Phospho-S6 Ribosomal protein (Ser235/236) antibody 80130-2-RR | Download protocol |
| WB protocol for Phospho-S6 Ribosomal protein (Ser235/236) antibody 80130-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Phospho-S6 Ribosomal protein (Ser235/236) recombinant monoclonal antibody (Cat# 80130-2-RR) has 3 citations. These appear in Nature Communications, Cell & Molecular Life Sciences, and International Journal of Molecular Sciences. The associated research fields span cancer biology, specifically pancreatic cancer pain signaling, endometrial cancer stemness, and triple-negative breast cancer phosphoproteomics. All three citations used the antibody for Western blot applications in human and/or mouse samples.
| Species | Application | Title |
|---|---|---|
Nat Commun Tumor-derived GDF15 induces CCN3⁺ Schwann cells to promote cancer pain in pancreatic cancer. | ||
Cell Mol Life Sci Phosphocholine inhibits proliferation and reduces stemness of endometrial cancer cells by downregulating mTOR-c-Myc signaling | ||
Int J Mol Sci SCAMP3-Driven Regulation of ERK1/2 and Autophagy Phosphoproteomics Signatures in Triple-Negative Breast Cancer. |
Reviews
What customers say
The single review for this product is highly positive (5/5). The reviewer used it for immunohistochemistry on FFPE human brain cortex sections at a 1:100 dilution and reported excellent performance, noting clear labeling of basal cells and dendrites in TSC tissue. Overall, the antibody is well-regarded for IHC applications on human brain tissue.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Reyes (Verified Customer) (02-10-2025) | pS6 worked perfectly in my FFPE human brain cortical sections. Marking my BCs and Dns in TSC tissue.
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