Phospho-Tau (Thr181) Polyclonal antibody

Phospho-Tau (Thr181) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28866-1-AP

Synonyms

MAPT, Neurofibrillary tangle protein, Paired helical filament tau, PHF tau, Phospho-TAU (Thr181), pThr181 Tau, TAU



Tested Applications

Positive WB detected inPDGF treated SH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

28866-1-AP targets Phospho-Tau (Thr181) in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name microtubule-associated protein tau
Calculated molecular weight 37-46, 79-81 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC000558
Gene symbol MAPT
Gene ID (NCBI) 4137
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tau (tubulin-associated unit) is a microtubule-associated protein (also known as MAPT), expressed mainly in neurons of the central nervous system. Its primary function is to modulate microtubule dynamics for maintaining axonal cytoskeleton. The Tau protein has six isoforms produced from a single gene through alternative RNA splicing. Isoforms differ in number of inserts at the N-terminal half and the number of repeats at the C-terminal half (3 repeat-3R; 4 repeat-4R). Tau is hyperphosphorylated during aging and in age-related neurodegenerative diseases such as Alzheimer's disease (AD) and fronto-temporal dementia. Hyperphosphorylation of Tau leads to the formation of neurofibrillary tangles (NFT) in the neurons and glia cells, which is one of the hallmarks of AD.

Protocols

Product Specific Protocols
WB protocol for Phospho-Tau (Thr181) antibody 28866-1-APDownload protocol
Standard Protocols
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