Tested Applications
| Positive WB detected in | PDGF treated SH-SY5Y cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 10 publications below |
| IF | See 1 publications below |
Product Information
28866-1-AP targets Phospho-TAU (Thr181) in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | microtubule-associated protein tau |
| Calculated Molecular Weight | 37-46, 79-81 kDa |
| Observed Molecular Weight | 50-80 kDa |
| GenBank Accession Number | BC000558 |
| Gene Symbol | TAU |
| Gene ID (NCBI) | 4137 |
| RRID | AB_2881222 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P10636 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Tau (tubulin-associated unit) is a microtubule-associated protein (also known as MAPT), expressed mainly in neurons of the central nervous system. Its primary function is to modulate microtubule dynamics for maintaining axonal cytoskeleton. The Tau protein has six isoforms produced from a single gene through alternative RNA splicing. Isoforms differ in number of inserts at the N-terminal half and the number of repeats at the C-terminal half (3 repeat-3R; 4 repeat-4R). Tau is hyperphosphorylated during aging and in age-related neurodegenerative diseases such as Alzheimer's disease (AD) and fronto-temporal dementia. Hyperphosphorylation of Tau leads to the formation of neurofibrillary tangles (NFT) in the neurons and glia cells, which is one of the hallmarks of AD.
Publications
| Species | Application | Title |
|---|---|---|
Biochim Biophys Acta Mol Basis Dis Apolipoprotein E ε4 triggers neurotoxicity via cholesterol accumulation, acetylcholine dyshomeostasis, and PKCε mislocalization in cholinergic neuronal cells | ||
Neural Plast Treatment Combining Focused Ultrasound with Gastrodin Alleviates Memory Deficit and Neuropathology in an Alzheimer's Disease-Like Experimental Mouse Model. | ||
Alzheimers Res Ther Uncovering the epigenetic regulatory clues of PRRT1 in Alzheimer's disease: a strategy integrating multi-omics analysis with explainable machine learning | ||
Neurochem Res Targeting UAF1 Alleviate Neurotoxicity by Inhibiting APP/NLRP3 Axis-Mediated Pyroptosis and Apoptosis | ||
Redox Biol Absence of astrocytic ceruloplasmin reverses the senescence process with aging of learning and memory abilities | ||
ACS Biomater Sci Eng RVG29 Peptide-Modified Exosomes Loaded with Mir-133b Mediate the RhoA-ROCK Pathway to Improve Motor and Neurological Symptoms in Parkinson's Disease |
