Tested Applications
| Positive WB detected in | Calyculin A treated HeLa cells |
| Positive FC (Intra) detected in | Calyculin A treated HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
Product Information
82382-2-RR targets Phospho-TBK1 (Ser172) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | TANK-binding kinase 1 |
| Observed Molecular Weight | 84 kDa |
| GenBank Accession Number | BC034950 |
| Gene Symbol | TBK1 |
| Gene ID (NCBI) | 29110 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q9UHD2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The serine/threonine protein kinase TBK1, discovered two decades ago as a member of the IκB kinase (IKK) protein kinase family, plays crucial roles in regulating innate immune response by promoting type I interferon (IFN) and modulating NF-κB signaling. The phosphorylation of Ser172 residue on TBK1 is critical and is commonly used as an indicator for its activity. (PMID: 39279883)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-TBK1 (Ser172) antibody 82382-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Clin Transl Med Elevation of ISG15 promotes diabetic kidney disease by modulating renal tubular epithelial cell pyroptosis | ||
Life Sci Improvement of the stability of ginsenoside Rg5 and its pharmacological efficacy in nonalcoholic steatohepatitis | ||
Int J Mol Sci Network Pharmacology, Molecular Dynamics Simulation, and Biological Validation Insights into the Potential of Ligustri Lucidi Fructus for Diabetic Nephropathy |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Marina (Verified Customer) (12-20-2025) | 1:500 dilution, overnight incubation. Higher molecular weight bands were observed (over 100 kDa). When the membrane was cut, subtle bands were observed at around 75 kDa. Ponceau staining was used as total protein loading control.
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