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Tested Applications
| Positive WB detected in | HEK-293 cells, λ phosphatase treated HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
80706-3-RR targets Phospho-c-SRC (Ser17) in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) |
| Calculated Molecular Weight | 60 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC011566 |
| Gene Symbol | SRC |
| Gene ID (NCBI) | 6714 |
| ENSEMBL Gene ID | ENSG00000197122 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P12931 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SRC, also named as SRC1 and p60-Src, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. The phosphorylation of c-SRC at Ser17 is essential for its activation. This phosphorylation event is often mediated by protein kinase A (PKA) and is crucial for downstream signaling events. (PMID: 23360994)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-c-SRC (Ser17) antibody 80706-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
