Tested Applications
| Positive WB detected in | RT-4 cells, U-87 MG cells, Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86386-3-RR targets RABGGTA in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag5923 Product name: Recombinant human RABGGTA protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 143-407 aa of BC003093 Sequence: FHCWDYRRFVATQAAVPPAEELAFTDSLITRNFSNYSSWHYRSCLLPQLHPQPDSGPQGRLPEDVLLKELELVQNAFFTDPNDQSAWFYHRWLLGRADPQDALRCLHVSRDEACLTVSFSRPLLVGSRMEILLLMVDDSPLIVEWRTPDGRNRPSHVWLCDLPAASLNDQLPQHTFRVIWTAGDVQKECVLLKGRQEGWCRDSTTDEQLFRCELSVEKSTVLQSELESCKELQELEPENKWCLLTIILLMRALDPLLYEKETLQY Predict reactive species |
| Full Name | Rab geranylgeranyltransferase, alpha subunit |
| Calculated Molecular Weight | 65 kDa |
| Observed Molecular Weight | 60~65 kDa |
| GenBank Accession Number | BC003093 |
| Gene Symbol | RABGGTA |
| Gene ID (NCBI) | 5875 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q92696 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GGTase II (RAB geranylgeranyl transferase, RABGGTase) is a heterodimer composed of tightly bound α- and β-subunits, which belongs to the Ras superfamily of small monomeric GTPases. RABGGTase is extensively involved in intracellular membrane trafficking of membrane-bound organelles and vesicles and specifically catalyses the covalent attachment of GGPP to the cysteine of a carboxyl-terminal CC or CXC motif of RAB proteins (PMID 11136552, 10737774, 35961942).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RABGGTA antibody 86386-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
