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RBPJ Monoclonal antibody

RBPJ Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

2F12G12

Cat no : 66132-1-Ig

Synonyms

CBF 1, CBF1, csl, IGKJRB, IGKJRB1, KBF2, NY-REN-30, RBP J, RBP J kappa, RBP JK, RBPJ, RBPJK, RBPSUH, SUH

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using RBPJ Monoclonal antibody (66132-1-Ig)

    WB analysis of HeLa using 66132-1-Ig

    WB result of RBPJ antibody (66132-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBPJ transfected HeLa cells.

  • Western Blot (WB) analysis of HepG2 cells using RBPJ Monoclonal antibody (66132-1-Ig)

    WB analysis of HepG2 using 66132-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66132-1-Ig (RBPJ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using RBPJ Monoclonal antibody (66132-1-Ig)

    WB analysis of human brain using 66132-1-Ig

    human brain tissue were subjected to SDS PAGE followed by western blot with 66132-1-Ig (RBPJ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHepG2 cells, human brain tissue, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 66132-1-Ig is RBPJ Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RBPJ fusion protein Ag6302
Full Name recombination signal binding protein for immunoglobulin kappa J region
Calculated Molecular Weight 56 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC064976
Gene Symbol RBPJ
Gene ID (NCBI) 3516
RRIDAB_2881531
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RBPJ antibody 66132-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols