Recoverin Polyclonal antibody

Recoverin Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10073-1-AP

Synonyms

Protein CAR, RCV1, RCVRN, recoverin



Tested Applications

Positive WB detected inmouse eye tissue, Y79 cells
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10073-1-AP targets Recoverin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recoverin fusion protein Ag0119
Full Name recoverin
Calculated molecular weight 24 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC001720
Gene symbol RCVRN
Gene ID (NCBI) 5957
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Recoverin, belonging to a family of the neuronal calcium sensor (NCS) proteins, has a restricted expression in retinal photoreceptors or neurons or neuroendocrine cells. It has been suggested to play a role in light and dark adaptation by regulating rhodopsin phosphorylation. Recently, it has been found that autoantibodies against recoverin (24 kDa) have been strongly associated with cancer -associated retinopathy (CAR) syndrome, a paraneoplastic disease of the retina. But functions of recoverin in cancer cells remain unknown.

Protocols

Product Specific Protocols
WB protocol for Recoverin antibody 10073-1-APDownload protocol
IF protocol for Recoverin antibody 10073-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Vet Ophthalmol

Evaluation of canine serum for the presence of antiretinal autoantibodies in sudden acquired retinal degeneration syndrome.

Authors - Keller Rachel L RL
ratIHC

Curr Eye Res

Transplanted olfactory ensheathing cells reduce retinal degeneration in Royal College of Surgeons rats.

Authors - Huo Shu Jia SJ
mouseIF

Sci Rep

Microglia dynamics in retinitis pigmentosa model: formation of fundus whitening and autofluorescence as an indicator of activity of retinal degeneration.

Authors - Kenichi Makabe
humanIF

Sci Rep

Preconditioning the Initial State of Feeder-free Human Pluripotent Stem Cells Promotes Self-formation of Three-dimensional Retinal Tissue.

Authors - Atsushi Kuwahara
ratWB

J Neurosci

Differentiation of marrow stromal cells into photoreceptors in the rat eye.

Authors - Kicic Anthony A