RELB Polyclonal antibody

RELB Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 25027-1-AP

Synonyms

I REL, IREL, RELB, Transcription factor RelB



Tested Applications

Positive WB detected inRamos cells
Positive IP detected inRaji cells
Positive IHC detected inhuman lymphoma tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25027-1-AP is RELB Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RELB fusion protein Ag13824
Full Name v-rel reticuloendotheliosis viral oncogene homolog B
Calculated molecular weight 62 kDa
Observed molecular weight 62 kDa
GenBank accession numberBC028013
Gene symbol RELB
Gene ID (NCBI) 5971
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RELB, also named as Transcription factor RelB, is a 579 amino acid protein, which contains one RHD domain. RELB localizes in the nucleus and cytoplasm. RELB is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis.

Protocols

Product Specific Protocols
WB protocol for RELB antibody 25027-1-APDownload protocol
IHC protocol for RELB antibody 25027-1-APDownload protocol
IF protocol for RELB antibody 25027-1-APDownload protocol
IP protocol for RELB antibody 25027-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

Identification of 11(13)-dehydroivaxillin as a potent therapeutic agent against non-Hodgkin's lymphoma.

Authors - Xinhua Xiao
humanWB, IHC

Nat Genet

Exome sequencing identifies somatic mutations of DDX3X in natural killer/T-cell lymphoma.

Authors - Lu Jiang