Tested Applications
| Positive WB detected in | Daudi cells, Raji cells, Ramos cells |
| Positive IP detected in | Raji cells |
| Positive IHC detected in | human colon cancer tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25027-1-AP targets RELB in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13824 Product name: Recombinant human RELB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 264-579 aa of BC028013 Sequence: NVVRICFQASYRDQQGQMRRMDPVLSEPVYDKKSTNTSELRICRINKESGPCTGGEELYLLCDKVQKEDISVVFSRASWEGRADFSQADVHRQIAIVFKTPPYEDLEIVEPVTVNVFLQRLTDGVCSEPLPFTYLPRDHDSYGVDKKRKRGMPDVLGELNSSDPHGIESKRRKKKPAILDHFLPNHGSGPFLPPSALLPDPDFFSGTVSLPGLEPPGGPDLLDDGFAYDPTAPTLFTMLDLLPPAPPHASAVVCSGGAGAVVGETPGPEPLTLDSYQAPGPGDGGTASLVGSNMFPNHYREAAFGGGLLSPGPEAT Predict reactive species |
| Full Name | v-rel reticuloendotheliosis viral oncogene homolog B |
| Calculated Molecular Weight | 62 kDa |
| Observed Molecular Weight | 62-65 kDa |
| GenBank Accession Number | BC028013 |
| Gene Symbol | RELB |
| Gene ID (NCBI) | 5971 |
| RRID | AB_2879856 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q01201 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RELB, also named as Transcription factor RelB, is a 579 amino acid protein, which contains one RHD domain. RELB localizes in the nucleus and cytoplasm. RELB is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RELB antibody 25027-1-AP | Download protocol |
| IHC protocol for RELB antibody 25027-1-AP | Download protocol |
| IP protocol for RELB antibody 25027-1-AP | Download protocol |
| WB protocol for RELB antibody 25027-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RELB Polyclonal antibody (Cat# 25027-1-AP) has 19 citations spanning journals including Cell, Nature Genetics, Nucleic Acids Research, Cell Death & Disease, Cell Chemical Biology, Stem Cells, and Journal of Clinical Endocrinology & Metabolism. Research fields covered include NF-κB signaling, ferroptosis, lymphoma, lung inflammation, hepatotoxicity, cancer metastasis, stem cell biology, and immune regulation.
| Species | Application | Title |
|---|---|---|
Nat Genet Exome sequencing identifies somatic mutations of DDX3X in natural killer/T-cell lymphoma. | ||
Nucleic Acids Res p38-mediated FOXN3 phosphorylation modulates lung inflammation and injury through the NF-κB signaling pathway | ||
Cell Death Dis Systems biology-enabled targeting of NF-κΒ and BCL2 overcomes microenvironment-mediated BH3-mimetic resistance in DLBCL | ||
Cell Death Dis Identification of 11(13)-dehydroivaxillin as a potent therapeutic agent against non-Hodgkin's lymphoma. | ||
Cell Chem Biol N-acetyltransferase 10 regulates chemokine expression in keratinocytes and promotes atopic dermatitis. |
Reviews
What customers say
Both reviewers report positive experiences with this antibody. One user (5★) used it for Western Blot at 1:2000 on HEK293 and U2OS cells, noting it works well when blocking with milk. Another (4★) tested it for IHC, IF, and Flow Cytometry at 1:100 on bladder epithelium, finding it performed well and planning to use it for in vivo experiments. Overall, users find it reliable across multiple applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Lisa (Verified Customer) (03-05-2026) | Nice working AB for Western blot if blocking with milk.
|
Laura (Verified Customer) (04-18-2025) | Antibody seemed to work well - will start using for my in vivo experiments
 |
