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  • Featured Product
  • KD/KO Validated

RNF2 Polyclonal antibody

RNF2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 16031-1-AP

Synonyms

HIP2 interacting protein 3, EC:2.3.2.27, E3 ubiquitin-protein ligase RING2, DING, BAP1

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using RNF2 Polyclonal antibody (16031-1-AP)

    WB analysis of K-562 using 16031-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 16031-1-AP (RNF2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using RNF2 Polyclonal antibody (16031-1-AP)

    WB analysis of human brain using 16031-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 16031-1-AP (RNF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using RNF2 Polyclonal antibody (16031-1-AP)

    WB analysis of U-937 using 16031-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 16031-1-AP (RNF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using RNF2 Polyclonal antibody (16031-1-AP)

    WB analysis of NIH/3T3 using 16031-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16031-1-AP (RNF2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using RNF2 Polyclonal antibody (16031-1-AP)

    IP experiment of K-562 using 16031-1-AP

    IP result of anti-RNF2 (IP:16031-1-AP, 4ug; Detection:16031-1-AP 1:800) with K-562 cells lysate 1200ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RNF2 Polyclonal antibody (16031-1-AP)

    IF Staining of HeLa using 16031-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 16031-1-AP (RNF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inK-562 cells, human brain tissue, NIH/3T3 cells, U-937 cells
Positive IP detected inK-562 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16031-1-AP targets RNF2 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNF2 fusion protein Ag8900
Full Name ring finger protein 2
Calculated Molecular Weight 336 aa, 38 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC012583
Gene Symbol RNF2
Gene ID (NCBI) 6045
RRIDAB_2180459
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RNF2 antibody 16031-1-APDownload protocol
IF protocol for RNF2 antibody 16031-1-APDownload protocol
IP protocol for RNF2 antibody 16031-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Cell

Coordinated regulation of polycomb group complexes through microRNAs in cancer.

Authors - Qi Cao
  • KD Validated
View Article
humanIHC

Cancer Cell

The Polycomb Repressor Complex 1 Drives Double-Negative Prostate Cancer Metastasis by Coordinating Stemness and Immune Suppression.

Authors - Wenjing Su
  • KD Validated
View Article

Dev Cell

Nucleoporin Seh1 controls murine neocortical development via transcriptional repression of p21 in neural stem cells

Authors - Wenxiu Dai
View Article
humanWB,IHC

FEBS J

Radiation resistance of cancer cells caused by mitochondrial dysfunction depends on SIRT3-mediated mitophagy

Authors - Yan Wei
View Article
humanWB, IF

Front Cell Dev Biol

RNF2 Mediates Hepatic Stellate Cells Activation by Regulating ERK/p38 Signaling Pathway in LX-2 Cells.

Authors - Qi Yan
  • KD Validated
View Article
humanWB

Front Oncol

Long non-coding RNA PRR7-AS1 promotes osteosarcoma progression via binding RNF2 to transcriptionally suppress MTUS1

Authors - Gu Chen-Xi
  • KD Validated
View Article