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HOIP Polyclonal antibody

HOIP Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16289-1-AP

Synonyms

HOIL 1 interacting protein, HOIP, ring finger protein 31, RNF31, ZIBRA

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using HOIP Polyclonal antibody (16289-1-AP)

    WB analysis of HeLa using 16289-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16289-1-AP (HOIP antibody at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Transfected cell cell using HOIP Polyclonal antibody (16289-1-AP)

    WB analysis of Transfected cell cell using 16289-1-AP

    WB result of RNF31/HOIP antibody (16289-1-AP) by Emmanuel Laplantine (Institute Pasteur,Paris,France).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HOIP Polyclonal antibody (16289-1-AP)

    IHC staining of human breast cancer using 16289-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16289-1-AP (HOIP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HOIP Polyclonal antibody (16289-1-AP)

    IHC staining of human breast cancer using 16289-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16289-1-AP (HOIP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, Transfected cell cell
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 5 publications below

Product Information

16289-1-AP targets HOIP in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HOIP fusion protein Ag9440
Full Name ring finger protein 31
Calculated Molecular Weight 1072 aa, 120 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC017376
Gene Symbol RNF31
Gene ID (NCBI) 55072
RRIDAB_2878239
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for HOIP antibody 16289-1-APDownload protocol
IHC protocol for HOIP antibody 16289-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncogene

AGO4 suppresses tumor growth by modulating autophagy and apoptosis via enhancing TRIM21-mediated ubiquitination of GRP78 in a p53-independent manner

Authors - Lin Wang
View Article
humanWB

Bioorg Med Chem

Biophysical and biological evaluation of optimized stapled peptide inhibitors of the linear ubiquitin chain assembly complex (LUBAC).

Authors - Francisco Aguilar-Alonso
View Article
humanWB

BMC Musculoskelet Disord

Polyglucosan body myopathy 1 may cause cognitive impairment: a case report from China.

Authors - Lin Chen
View Article
humanWB

Arch Oral Biol

A novel inhibitor of nuclear factor kappa-B kinase subunit gamma mutation identified in an incontinentia pigmenti patient with syndromic tooth agenesis.

Authors - Shichen Sun
View Article
humanWB

Cancer Commun (Lond)

USP51/PD-L1/ITGB1-deployed juxtacrine interaction plays a cell-intrinsic role in promoting chemoresistant phenotypes in non-small cell lung cancer

Authors - Jianjun Li
View Article