MST4 Antibody

MST4 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10847-1-AP

Synonyms

MASK, MST 4, Mst3 and SOK1 related kinase, MST4, RP6 213H19.1, RP6-213H19.1, STE20 like kinase MST4



Tested Applications

Positive WB detected inMCF7 cells, HL-60 cells, Jurkat cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10847-1-AP targets MST4 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MST4 fusion protein Ag1302
Full Name serine/threonine protein kinase MST4
Calculated molecular weight 16 kDa,46 kDa
Observed molecular weight 47-50 kDa
GenBank accession number BC017213
Gene symbol RP6-213H19.1
Gene ID (NCBI) 51765
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MST4 antibody 10847-1-APDownload protocol
IHC protocol for MST4 antibody 10847-1-APDownload protocol
IF protocol for MST4 antibody 10847-1-APDownload protocol
IP protocol for MST4 antibody 10847-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Behav Neurol

MST4 Kinase Inhibitor Hesperadin Attenuates Autophagy and Behavioral Disorder via the MST4/AKT Pathway in Intracerebral Hemorrhage Mice.

Authors - Xiaodong Wu