Tested Applications
| Positive WB detected in | HEK-293 cells, K-562 cells, Y79 cells, A431 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12448-1-AP targets RPA1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3067 Product name: Recombinant human RPA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 317-616 aa of BC018126 Sequence: VDIIGICKSYEDATKITVRSNNREVAKRNIYLMDTSGKVVTATLWGEDADKFDGSRQPVLAIKGARVSDFGGRSLSVLSSSTIIANPDIPEAYKLRGWFDAEGQALDGVSISDLKSGGVGGSNTNWKTLYEVKSENLGQGDKPDYFSSVATVVYLRKENCMYQACPTQDCNKKVIDQQNGLYRCEKCDTEFPNFKYRMILSVNIADFQENQWVTCFQESAEAILGQNAAYLGELKDKNEQAFEEVFQNANFRSFIFRVRVKVETYNDESRIKATVMDVKPVDYREYGRRLVMSIRRSALM Predict reactive species |
| Full Name | replication protein A1, 70kDa |
| Calculated Molecular Weight | 616 aa, 68 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC018126 |
| Gene Symbol | RPA1 |
| Gene ID (NCBI) | 6117 |
| RRID | AB_2180495 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P27694 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Replication protein A 1 (RPA1) is a subunit of a stable heterotrimer replication protein A (RPA), which is conserved in almost all eukaryotic species (PMID: 27151292). Usually, RPA1, RPA2 and RPA3 function as a whole to participate in DNA replication, telomere maintenance, DNA recombination, DNA repair, and activation of DNA damage checkpoints. In the cell, RPA has a higher affinity to bind single strand DNA and then to form a fork protection complex with other components such as PTEN and OTUB1, thus maintains genome stability during replication(PMID: 26403191; PMID: 18469000).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RPA1 antibody 12448-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RPA1 Polyclonal antibody (Cat# 12448-1-AP) has 3 citations. They appear in Frontiers in Oncology, Experimental and Therapeutic Medicine, and npj Precision Oncology. The cited research spans lung cancer (cisplatin sensitivity via nucleotide excision repair), glioma progression, and ovarian cancer (BRCA-proficient, synthetic lethality with olaparib), all involving DNA replication/repair mechanisms.
| Species | Application | Title |
|---|---|---|
NPJ Precis Oncol HAMNO and CGK733 as BRCAness-driven candidates exhibit synthetic lethality with olaparib in BRCA-proficient ovarian cancer. |
