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RPL21 Polyclonal antibody

RPL21 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15226-1-AP

Synonyms

60S ribosomal protein L21, L21, ribosomal protein L21, RPL21

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using RPL21 Polyclonal antibody (15226-1-AP)

    WB analysis of HepG2 using 15226-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15226-1-AP (RPL21 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RPL21 Polyclonal antibody (15226-1-AP)

    WB analysis of HeLa using 15226-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15226-1-AP (RPL21 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using RPL21 Polyclonal antibody (15226-1-AP)

    WB analysis of HepG2 using 15226-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15226-1-AP (RPL21 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using RPL21 Polyclonal antibody (15226-1-AP)

    IHC staining of human liver using 15226-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 15226-1-AP (RPL21 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using RPL21 Polyclonal antibody (15226-1-AP)

    IHC staining of human liver using 15226-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 15226-1-AP (RPL21 antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15226-1-AP targets RPL21 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPL21 fusion protein Ag7444
Full Name ribosomal protein L21
Calculated Molecular Weight 19 kDa
Observed Molecular Weight 20-25 kDa
GenBank Accession NumberBC001603
Gene Symbol RPL21
Gene ID (NCBI) 6144
RRIDAB_2181591
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RPL21 antibody 15226-1-APDownload protocol
IHC protocol for RPL21 antibody 15226-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

iScience

TIAR and FMRP shape pro-survival nascent proteome of leukemia cells in the bone marrow microenvironment

Authors - Magdalena Wolczyk
View Article