RRN3 Polyclonal antibody

RRN3 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, chIP, ELISA

Conjugate

Unconjugated

Cat no : 25918-1-AP

Synonyms

RRN3, TIF IA, TIFIA



Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, K-562 cells, mouse testis tissue
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Published Applications

ChIPSee 1 publications below

Product Information

The immunogen of 25918-1-AP is RRN3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RRN3 fusion protein Ag23207
Full Name RRN3 RNA polymerase I transcription factor homolog (S. cerevisiae)
Observed molecular weight 74 kDa
GenBank accession numberBC132688
Gene symbol RRN3
Gene ID (NCBI) 54700
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RRN3 antibody 25918-1-APDownload protocol
IP protocol for RRN3 antibody 25918-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanchIP

Biochem Biophys Res Commun

Nucleolar localization signal and histone methylation reader function is required for SPIN1 to promote rRNA gene expression.

Authors - Xiaolei Zhang