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RRN3 Polyclonal antibody

RRN3 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, chIP, ELISA

Conjugate

Unconjugated

Cat no : 25918-1-AP

Synonyms

RRN3, TIF IA, TIFIA

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using RRN3 Polyclonal antibody (25918-1-AP)

    WB analysis of HeLa using 25918-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25918-1-AP (RRN3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using RRN3 Polyclonal antibody (25918-1-AP)

    WB analysis of Jurkat using 25918-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25918-1-AP (RRN3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using RRN3 Polyclonal antibody (25918-1-AP)

    WB analysis of K-562 using 25918-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 25918-1-AP (RRN3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using RRN3 Polyclonal antibody (25918-1-AP)

    WB analysis of mouse testis using 25918-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25918-1-AP (RRN3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RRN3 Polyclonal antibody (25918-1-AP)

    WB analysis of HeLa using 25918-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25918-1-AP (RRN3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using RRN3 Polyclonal antibody (25918-1-AP)

    WB analysis of mouse testis using 25918-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25918-1-AP (RRN3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using RRN3 Polyclonal antibody (25918-1-AP)

    IP experiment of HeLa using 25918-1-AP

    IP result of anti-RRN3 (IP:25918-1-AP, 4ug; Detection:25918-1-AP 1:500) with HeLa cells lysate 3200ug.

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, K-562 cells, mouse testis tissue
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25918-1-AP is RRN3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RRN3 fusion protein Ag23207
Full Name RRN3 RNA polymerase I transcription factor homolog (S. cerevisiae)
Observed Molecular Weight 74 kDa
GenBank Accession NumberBC132688
Gene Symbol RRN3
Gene ID (NCBI) 54700
RRIDAB_2880296
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RRN3 antibody 25918-1-APDownload protocol
IP protocol for RRN3 antibody 25918-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanchIP

Biochem Biophys Res Commun

Nucleolar localization signal and histone methylation reader function is required for SPIN1 to promote rRNA gene expression.

Authors - Xiaolei Zhang
View Article
WB

Mol Cell

Control of ribosomal RNA synthesis by hematopoietic transcription factors

Authors - Charles Antony
View Article