Tested Applications
| Positive WB detected in | PC-3 cells, Saos-2 cells, C2C12 cells |
| Positive IP detected in | ROS1728 cells |
| Positive ChIP-qPCR detected in | Saos-2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| CHIP-QPCR | CHIP-QPCR : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
82636-2-RR targets RUNX2 in WB, IHC, IF, IP, CoIP, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | runt-related transcription factor 2 |
| Calculated Molecular Weight | 57 kDa |
| Observed Molecular Weight | 57-60 kDa |
| GenBank Accession Number | NM_004348 |
| Gene Symbol | RUNX2 |
| Gene ID (NCBI) | 860 |
| RRID | AB_3086495 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q13950 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RUNX2, also named as AML3, CBFA1, OSF2 and PEBP2A, is a Transcription factor which involved in osteoblastic differentiation and skeletal morphogenesis. It is essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters By similarity. RUNX2 inhibits MYST4-dependent transcriptional activation.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for RUNX2 antibody 82636-2-RR | Download protocol |
| WB protocol for RUNX2 antibody 82636-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Int Immunopharmacol Aging and autophagic phenotypic changes in bone marrow mesenchymal stem cells in glucocorticoid-induced osteonecrosis | ||
Intractable Rare Dis Res Role of IFITM2 in osteogenic differentiation of C3H10T1/2 mesenchymal stem cells | ||
Bioact Mater Designed bone-targeting ROS-responsive nanoplatform for precision glycolysis inhibition in postmenopausal osteoporosis. | ||
Int J Nanomedicine MgFe-Layered Double Hydroxide-Reinforced Injectable GelMA Hydrogels Promote Calvarial Bone Regeneration with IL-10RA-JAK1-STAT3-Associated Osteoimmunomodulation. | ||
Biomaterials A ZIF90-tungsten-based nanoparticle hydrogel system with antibacterial and antioxidant properties promotes periodontal bone regeneration by modulating the ROS/Plagl1/Wnt pathway. | ||
Int J Pharm Regulation of macrophage-mediated osteogenesis by kaempferol liposomes in trauma-induced heterotopic ossification |
Reviews
What customers say
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Charlotte (Verified Customer) (07-22-2024) | I used this antibody in WB in cells that differentiate into osteoblast lineage upon stimulation. I had a strong visible band at the expected size
 |
Udesh (Verified Customer) (11-26-2023) | Worked well for ADSCs at 1:1000
|
