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  • KD/KO Validated

S100A10 Antibody

S100A10 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 11250-1-AP

Synonyms

42C, ANX2L, ANX2LG, CAL1L, Calpactin 1 light chain, Calpactin I light chain, Cellular ligand of annexin II, CLP11, GP11, p10, p10 protein, P11, Protein S100 A10, S100A10



Tested Applications

Positive WB detected inmouse lung tissue, human lung tissue, mouse small intestine tissue, rat skin tissue
Positive IHC detected inhuman lung cancer tissue, mouse lung tissue, human skin tissue, human pancreas cancer tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11250-1-AP targets S100A10 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen S100A10 fusion protein Ag1779
Full Name S100 calcium binding protein A10
Calculated molecular weight 11 kDa
Observed molecular weight 11 kDa
GenBank accession number BC015973
Gene symbol S100A10
Gene ID (NCBI) 6281
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

S100A10, also known as p11, is a member of the S100 family of small, EF hand containing dimeric proteins. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A10 is present on the surface of endothelial and other cells in a heterotetrameric complex with another Ca(2+)-binding protein, annexin II. S100A10 may function in exocytosis and endocytosis.

Protocols

Product Specific Protocols
WB protocol for S100A10 antibody 11250-1-APDownload protocol
IHC protocol for S100A10 antibody 11250-1-APDownload protocol
IF protocol for S100A10 antibody 11250-1-APDownload protocol
FC protocol for S100A10 antibody 11250-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Biosci Trends

Label-free quantification proteomics reveals novel calcium binding proteins in matrix vesicles isolated from mineralizing Saos-2 cells.

Authors - Zhou Xiaoying X
humanWB

Proteome Sci

Proteomic analysis of human periodontal ligament cells under hypoxia.

Authors - Qiwen Li
humanWB,IF

J Biosci

Down-regulation effects of IFN-α on p11, 5-htr1b and 5-HTR4 protein levels were affected by NH4CL or MG132 treatment in SH-sy5y cells.

Authors - Jiqiang Guo
  • KD Validated
humanWB,IP

J Biol Chem

Feedback regulation of endothelial cell surface plasmin generation by PKC-dependent phosphorylation of annexin A2.

Authors - He Kai-Li KL
humanWB,IHC

Mol Oncol

S100A10, a Novel Biomarker in Pancreatic Ductal Adenocarcinoma.

Authors - Moamen Bydoun
human,mouseWB,IHC

Sci Rep

Probable involvement of p11 with interferon alpha induced depression.

Authors - Jiqiang Guo