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S100A11 Polyclonal antibody

S100A11 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10237-1-AP

Synonyms

Calgizzarin, MLN 70, MLN70, Protein S100 A11, Protein S100 C, S100A11, S100C



Tested Applications

Positive WB detected inHCT 116 cells, PC-3 cells, HEK-293 cells, human lung tissue, DU 145 cells, human heart tissue, COLO 320 cells, BxPC-3 cells, PC-12 cells, SKOV-3 cells, U2OS cells, HaCaT cells
Positive IP detected inPC-3 cells
Positive IHC detected inhuman breast cancer tissue, mouse liver tissue, human ovary tumor tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10237-1-AP targets S100A11 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen S100A11 fusion protein Ag0343
Full Name S100 calcium binding protein A11
Calculated molecular weight 105 aa, 12 kDa
Observed molecular weight 12 kDa
GenBank accession numberBC001410
Gene symbol S100A11
Gene ID (NCBI) 6282
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

S100A11 (also known as S100C or calziggarin), is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs (PMID: 18694925). S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. First discovered in 1989, S100A11 has since been proposed to have direct biological functions in an assortment of physiological processes such as endo- and exocytosis, regulation of enzyme activity, cell growth and regulation, apoptosis and inflammation (PMID: 15241500). Chromosomal rearrangements and altered expression of S100A11 have been implicated in tumor metastasis. This S100A11 antibody (10237-1-AP) has also been instrumental in investigations into S100A11's role in the development of brain tumors in tuberous sclerosis complex (TSC) patients (PMID: 20133820).

Protocols

Product Specific Protocols
WB protocol for S100A11 antibody 10237-1-APDownload protocol
IHC protocol for S100A11 antibody 10237-1-APDownload protocol
IP protocol for S100A11 antibody 10237-1-APDownload protocol
FC protocol for S100A11 antibody 10237-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Rep

Annexin A7 is required for ESCRT III-mediated plasma membrane repair.

Authors - Stine Lauritzen Sønder
humanWB,IF

Biochim Biophys Acta

Disruption of the annexin A1/S100A11 complex increases the migration and clonogenic growth by dysregulating epithelial growth factor (EGF) signaling.

Authors - Poeter Michaela M
humanWB,IHC

Hepatobiliary Pancreat Dis Int

Comparative proteomic analysis of differentially expressed proteins in human pancreatic cancer tissue.

Authors - Chen Jian-Hua JH
humanWB

Oncol Lett

Apigenin inhibits growth and induces apoptosis in human cholangiocarcinoma cells.

Authors - Pantipa Subhasitanont
humanWB

Int J Med Sci

S100A11 Is a Migration-Related Protein in Laryngeal Squamous Cell Carcinoma.

Authors - Wang Chengyu C
humanWB,IHC

Int J Colorectal Dis

Colorectal cancer progression correlates with upregulation of S100A11 expression in tumor tissues.

Authors - Wang Guiyu G