|Positive WB detected in||HCT 116 cells, PC-3 cells, HEK-293 cells, human lung tissue, DU 145 cells, human heart tissue, COLO 320 cells, BxPC-3 cells, PC-12 cells, SKOV-3 cells, U2OS cells, HaCaT cells|
|Positive IP detected in||PC-3 cells|
|Positive IHC detected in||human breast cancer tissue, mouse liver tissue, human ovary tumor tissue, human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10237-1-AP targets S100A11 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, rat, mouse samples.
|Tested Reactivity||human, rat, mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||S100A11 fusion protein Ag0343|
|Full Name||S100 calcium binding protein A11|
|Calculated molecular weight||105 aa, 12 kDa|
|Observed molecular weight||12 kDa|
|GenBank accession number||BC001410|
|Gene ID (NCBI)||6282|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
S100A11 (also known as S100C or calziggarin), is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs (PMID: 18694925). S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. First discovered in 1989, S100A11 has since been proposed to have direct biological functions in an assortment of physiological processes such as endo- and exocytosis, regulation of enzyme activity, cell growth and regulation, apoptosis and inflammation (PMID: 15241500). Chromosomal rearrangements and altered expression of S100A11 have been implicated in tumor metastasis. This S100A11 antibody (10237-1-AP) has also been instrumental in investigations into S100A11's role in the development of brain tumors in tuberous sclerosis complex (TSC) patients (PMID: 20133820).
|Product Specific Protocols|
|WB protocol for S100A11 antibody 10237-1-AP||Download protocol|
|IHC protocol for S100A11 antibody 10237-1-AP||Download protocol|
|IP protocol for S100A11 antibody 10237-1-AP||Download protocol|
|FC protocol for S100A11 antibody 10237-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Annexin A7 is required for ESCRT III-mediated plasma membrane repair.
Biochim Biophys Acta
Disruption of the annexin A1/S100A11 complex increases the migration and clonogenic growth by dysregulating epithelial growth factor (EGF) signaling.
Hepatobiliary Pancreat Dis Int
Comparative proteomic analysis of differentially expressed proteins in human pancreatic cancer tissue.
Apigenin inhibits growth and induces apoptosis in human cholangiocarcinoma cells.
Int J Med Sci
S100A11 Is a Migration-Related Protein in Laryngeal Squamous Cell Carcinoma.
Int J Colorectal Dis
Colorectal cancer progression correlates with upregulation of S100A11 expression in tumor tissues.