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SAPS1 Polyclonal antibody

SAPS1 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17819-1-AP

Synonyms

KIAA1115, PP6R1, PPP6R1, SAP190, SAPS domain family member 1, SAPS domain family, member 1, SAPS1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using SAPS1 Polyclonal antibody (17819-1-AP)

    WB analysis of HeLa using 17819-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17819-1-AP (SAPS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using SAPS1 Polyclonal antibody (17819-1-AP)

    WB analysis of HEK-293 using 17819-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 17819-1-AP (SAPS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using SAPS1 Polyclonal antibody (17819-1-AP)

    WB analysis of mouse testis using 17819-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 17819-1-AP (SAPS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using SAPS1 Polyclonal antibody (17819-1-AP)

    IP experiment of HeLa using 17819-1-AP

    IP result of anti-SAPS1 (IP:17819-1-AP, 4ug; Detection:17819-1-AP 1:1000) with HeLa cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human testis tissue using SAPS1 Polyclonal antibody (17819-1-AP)

    IHC staining of human testis using 17819-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17819-1-AP (SAPS1 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using SAPS1 Polyclonal antibody (17819-1-AP)

    IHC staining of human testis using 17819-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17819-1-AP (SAPS1 antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, mouse testis tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IPSee 1 publications below

Product Information

17819-1-AP targets SAPS1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SAPS1 fusion protein Ag12149
Full Name SAPS domain family, member 1
Calculated Molecular Weight881aa,97 kDa; 943aa,103 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC094753
Gene Symbol SAPS1
Gene ID (NCBI) 22870
RRIDAB_2183809
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SAPS1 antibody 17819-1-APDownload protocol
IHC protocol for SAPS1 antibody 17819-1-APDownload protocol
IP protocol for SAPS1 antibody 17819-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIP

Cell Death Dis

PP6 negatively modulates LUBAC-mediated M1-ubiquitination of RIPK1 and c-FLIPL to promote TNFα-mediated cell death

Authors - Guowei Wu
View Article