Tested Applications
| Positive IF/ICC detected in | HEK-293T cells |
| Positive ELISA detected in | N/A |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| Enzyme-linked Immunosorbent Assay (ELISA) | ELISA : |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29286-1-AP targets SARS-COV-2 NSP5 in WB, IF/ICC, ELISA applications and shows reactivity with virus samples.
| Tested Reactivity | virus |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag30934 Product name: Recombinant sars-cov-2 NSP5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-306 aa of YP_009742612 Sequence: SGFRKMAFPSGKVEGCMVQVTCGTTTLNGLWLDDVVYCPRHVICTSEDMLNPNYEDLLIRKSNHNFLVQAGNVQLRVIGHSMQNCVLKLKVDTANPKTPKYKFVRIQPGQTFSVLACYNGSPSGVYQCAMRPNFTIKGSFLNGSCGSVGFNIDYDCVSFCYMHHMELPTGVHAGTDLEGNFYGPFVDRQTAQAAGTDTTITVNVLAWLYAAVINGDRWFLNRFTTTLNDFNLVAMKYNYEPLTQDHVDILGPLSAQTGIAVLDMCASLKELLQNGMNGRTILGSALLEDEFTPFDVVRQCSGVTFQ Predict reactive species |
| Full Name | ORF1ab |
| GenBank Accession Number | YP_009742612 |
| Gene Symbol | SARS-COV-2 NSP8 |
| Gene ID (NCBI) | 43740578 |
| RRID | AB_2918269 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Publications
What published studies show
The SARS-CoV-2 NSP5 Polyclonal Antibody (Cat# 29286-1-AP) has 3 total citations. These appear in Cell Reports, Journal of Cell Biology, and Journal of Biological Chemistry. The cited research spans virology and host-pathogen interactions, specifically addressing SARS-CoV-2 replication organelle formation, viral protease MPro ubiquitination by ZBTB25, and the interplay between mitophagy, IFN signaling, and viral evolution. Applications cited include WB and IF in human samples.
| Species | Application | Title |
|---|---|---|
Cell Rep Pressure to evade cell-autonomous innate sensing reveals interplay between mitophagy, IFN signaling, and SARS-CoV-2 evolution | ||
J Cell Biol SARS-CoV-2 NSP3/4 control formation of replication organelle and recruitment of RNA polymerase NSP12 | ||
J Biol Chem E3 ubiquitin ligase ZBTB25 suppresses beta coronavirus infection through ubiquitination of the main viral protease MPro |
