Secretogranin II Polyclonal antibody
Secretogranin II Polyclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF,ELISA
Conjugate
Unconjugated
Cat no : 20357-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | SH-SY5Y cells |
Positive IHC detected in | mouse pancreas tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | mouse pancreas tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
The immunogen of 20357-1-AP is Secretogranin II Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Secretogranin II fusion protein Ag14215 |
Full Name | secretogranin II (chromogranin C) |
Calculated molecular weight | 617 aa, 71 kDa |
Observed molecular weight | 71 kDa |
GenBank accession number | BC022509 |
Gene symbol | SCG2 |
Gene ID (NCBI) | 7857 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SCG2, also known as secretogranin II or Chromogranin C, is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is widely distributed in nervous and endocrine tissues, and abundant in neuroendocrine granules. In the brain secretogranin II is almost completely processed to secretoneurin, a peptide involved in neurite outgrowth, neuroprotection and neuronal plasticity.
Protocols
Product Specific Protocols | |
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WB protocol for Secretogranin II antibody 20357-1-AP | Download protocol |
IHC protocol for Secretogranin II antibody 20357-1-AP | Download protocol |
IF protocol for Secretogranin II antibody 20357-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |