Tested Applications
| Positive WB detected in | SH-SY5Y cells |
| Positive IHC detected in | mouse pancreas tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse pancreas tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 8 publications below |
| IHC | See 6 publications below |
| IF | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
20357-1-AP targets Secretogranin II in WB, IHC, IF-P, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Secretogranin II fusion protein Ag14215 Predict reactive species |
| Full Name | secretogranin II (chromogranin C) |
| Calculated Molecular Weight | 617 aa, 71 kDa |
| Observed Molecular Weight | 71 kDa |
| GenBank Accession Number | BC022509 |
| Gene Symbol | Chromogranin C/SGII |
| Gene ID (NCBI) | 7857 |
| RRID | AB_10699881 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P13521 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SCG2, also known as secretogranin II or Chromogranin C, is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is widely distributed in nervous and endocrine tissues, and abundant in neuroendocrine granules. In the brain secretogranin II is almost completely processed to secretoneurin, a peptide involved in neurite outgrowth, neuroprotection and neuronal plasticity.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Secretogranin II antibody 20357-1-AP | Download protocol |
| IHC protocol for Secretogranin II antibody 20357-1-AP | Download protocol |
| IF protocol for Secretogranin II antibody 20357-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Rep Gut microbiota shapes social dominance through modulating HDAC2 in the medial prefrontal cortex. | ||
J Cell Biol SNX5 targets a monoamine transporter to the TGN for assembly into dense core vesicles by AP-3. | ||
Mol Oncol Secretogranin II impairs tumor growth and angiogenesis by promoting degradation of hypoxia-inducible factor-1α in colorectal cancer. | ||
Front Mol Biosci Tumor Microenvironment Heterogeneity-Based Score System Predicts Clinical Prognosis and Response to Immune Checkpoint Blockade in Multiple Colorectal Cancer Cohorts. | ||
Front Physiol Potential biomarkers and immune cell infiltration involved in aortic valve calcification identified through integrated bioinformatics analysis | ||
Front Neurol Tandem mass tag-based quantitative proteomic analysis of effects of multiple sevoflurane exposures on the cerebral cortex of neonatal and adult mice |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Wojciech (Verified Customer) (03-02-2023) | The antibody worked very well. The dilution used was 1:1000 in 5% non-fat milk. The single detected band size was around 75 kDa.
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