Live Chat

Secretogranin II Polyclonal antibody

Secretogranin II Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 20357-1-AP

Synonyms

CHGC, Chromogranin C, SCG2, Secretogranin 2, Secretogranin II, SgII, SN

Validation Data Gallery

  • WB analysis using 20357-1-AP

    WB analysis using 20357-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20357-1-AP (Secretogranin II antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse pancreas using 20357-1-AP

    IHC staining of mouse pancreas using 20357-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 20357-1-AP (Secretogranin II antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse pancreas using 20357-1-AP

    IF Staining of mouse pancreas using 20357-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using 20357-1-AP (Secretogranin II antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inSH-SY5Y cells
Positive IHC detected inmouse pancreas tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse pancreas tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20357-1-AP is Secretogranin II Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Secretogranin II fusion protein Ag14215
Full Name secretogranin II (chromogranin C)
Calculated molecular weight 617 aa, 71 kDa
Observed molecular weight 71 kDa
GenBank accession numberBC022509
Gene symbol SCG2
Gene ID (NCBI) 7857
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SCG2, also known as secretogranin II or Chromogranin C, is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is widely distributed in nervous and endocrine tissues, and abundant in neuroendocrine granules. In the brain secretogranin II is almost completely processed to secretoneurin, a peptide involved in neurite outgrowth, neuroprotection and neuronal plasticity.

Protocols

Product Specific Protocols
WB protocol for Secretogranin II antibody 20357-1-APDownload protocol
IHC protocol for Secretogranin II antibody 20357-1-APDownload protocol
IF protocol for Secretogranin II antibody 20357-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols