Secretogranin II Polyclonal antibody

Secretogranin II Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 20357-1-AP

Synonyms

CHGC, Chromogranin C, SCG2, Secretogranin 2, Secretogranin II, SgII, SN



Tested Applications

Positive WB detected inSH-SY5Y cells
Positive IHC detected inmouse pancreas tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse pancreas tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20357-1-AP targets Secretogranin II in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Secretogranin II fusion protein Ag14215
Full Name secretogranin II (chromogranin C)
Calculated Molecular Weight 617 aa, 71 kDa
Observed Molecular Weight 71 kDa
GenBank Accession NumberBC022509
Gene Symbol SCG2
Gene ID (NCBI) 7857
RRIDAB_10699881
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SCG2, also known as secretogranin II or Chromogranin C, is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is widely distributed in nervous and endocrine tissues, and abundant in neuroendocrine granules. In the brain secretogranin II is almost completely processed to secretoneurin, a peptide involved in neurite outgrowth, neuroprotection and neuronal plasticity.

Protocols

Product Specific Protocols
WB protocol for Secretogranin II antibody 20357-1-APDownload protocol
IHC protocol for Secretogranin II antibody 20357-1-APDownload protocol
IF protocol for Secretogranin II antibody 20357-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Cell Rep

Gut microbiota shapes social dominance through modulating HDAC2 in the medial prefrontal cortex.

Authors - Tian Wang
mouse,ratWB,IF

J Cell Biol

SNX5 targets a monoamine transporter to the TGN for assembly into dense core vesicles by AP-3.

Authors - Hongfei Xu
humanWB,IHC,CoIP

Mol Oncol

Secretogranin II impairs tumor growth and angiogenesis by promoting degradation of hypoxia-inducible factor-1α in colorectal cancer.

Authors - Chao Fang
humanIHC

Front Mol Biosci

Tumor Microenvironment Heterogeneity-Based Score System Predicts Clinical Prognosis and Response to Immune Checkpoint Blockade in Multiple Colorectal Cancer Cohorts.

Authors - Hufei Wang
humanIHC

Front Physiol

Potential biomarkers and immune cell infiltration involved in aortic valve calcification identified through integrated bioinformatics analysis

Authors - Xiaoshuo Lv
mouseWB

Front Neurol

Tandem mass tag-based quantitative proteomic analysis of effects of multiple sevoflurane exposures on the cerebral cortex of neonatal and adult mice

Authors - Jingyu Feng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Wojciech (Verified Customer) (03-02-2023)

The antibody worked very well. The dilution used was 1:1000 in 5% non-fat milk. The single detected band size was around 75 kDa.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: T-ALL cell lines