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SCRIB Polyclonal antibody

SCRIB Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27083-1-AP

Synonyms

CRIB1, hScrib, KIAA0147, LAP4, Protein LAP4, Protein scribble homolog, SCRB1, SCRIB, SCRIB1, Scribble, scribbled homolog (Drosophila), Vartul

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using SCRIB Polyclonal antibody (27083-1-AP)

    WB analysis using 27083-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 27083-1-AP (SCRIB antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 27083-1-AP is SCRIB Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SCRIB fusion protein Ag25795
Full Name scribbled homolog (Drosophila)
Calculated Molecular Weight 1630 aa, 175 kDa
Observed Molecular Weight 260 kDa
GenBank Accession NumberBC036905
Gene Symbol SCRIB
Gene ID (NCBI) 23513
RRIDAB_2880749
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SCRIB encodes a large, 260-kDa cytoplasmic scaffolding protein that comprises a large leucine-rich repeat (LRR) region and 4 PDZ domains that regulate protein-protein interactions. In mammals, cell polarity is established and maintained by at least 3 protein modules (Scrib, Crumbs, and Par). The apical (Crumbs and Par) and basolateral (Scrib) modules function in a mutually antagonistic relationship to regulate various polarization processes such as apical-basal polarity, planar cell polarity, asymmetric cell division and migration. The loss of SCRIB in malignant tumors suggest that it has potential to be a tumor suppressor. Alteration of polarity through deletion, downregulation, overexpression, and mislocalization can induce structural and functional alteration of cells that might be related to tumorigenesis. Mislocalization of SCRIB is involved in epithelial-to-mesenchymal transition (EMT) by inducing loss of E-cadherin. Deregulated SCRIB expression induces tumorigenesis in breast and liver. (PMID: 28460446, PMID: 32564009)

Protocols

Product Specific Protocols
WB protocol for SCRIB antibody 27083-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Oxid Med Cell Longev

MiR-9-5p Inhibits the MMP+-Induced Neuron Apoptosis through Regulating SCRIB/β-Catenin Signaling in Parkinson's Disease.

Authors - Zhenyong Xiao
View Article