Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, mouse brain tissue |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
14303-1-AP targets SCRN1 in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SCRN1 fusion protein Ag5413 Predict reactive species |
| Full Name | secernin 1 |
| Calculated Molecular Weight | 46 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | BC040492 |
| Gene Symbol | SCRN1 |
| Gene ID (NCBI) | 9805 |
| RRID | AB_2878043 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q12765 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SCRN genes encode protein members of the secernin family including Secernin 1, Secernin 2 and Secernin 3. Function of Secernin 2 and Secernin 3 are so far not well understood. Secernin 1 is a novel 50-kDa cytosolic protein that appears to be involved in the regulation of exocytosis from peritoneal mast cells. Secernin-1 a novel tumor-associated antigen (TAA) and may be a universal marker of different cancer types including gastric cancer, which has been validated by several researches. Measurement of SCRN1 can be used in the early detection of cancer or in the surveillance of patients who undergo surgery.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SCRN1 antibody 14303-1-AP | Download protocol |
| IHC protocol for SCRN1 antibody 14303-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Mol Neurosci Integrated proteomics and metabolomics analysis of D-pinitol function during hippocampal damage in streptozocin-induced aging-accelerated mice | ||
Biochem Biophys Res Commun Proteomic profiling of brain cortex tissues in a Tau transgenic mouse model of Alzheimer's disease. | ||
