Tested Applications
| Positive IHC detected in | human tonsillitis tissue, human lymphoma tissue, human appendicitis tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:4000-1:16000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67155-1-Ig targets CD138/Syndecan-1 in IHC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0823 Product name: Recombinant human SDC1,CD138 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-310 aa of BC008765 Sequence: MRRAALWLWLCALALSLQPALPQIVATNLPPEDQDGSGDDSDNFSGSGAGALQDITLSQQTPSTWKDTQLLTAIPTSPEPTGLEATAASTSTLPAGEGPKEGEAVVLPEVEPGLTAREQEATPRPRETTQLPTTHQASTTTATTAQEPATSHPHRDMQPGHHETSTPAGPSQADLHTPHTEDGGPSATERAAEDGASSQLPAAEGSGEQDFTFETSGENTAVVAVEPDRRNQSPVDQGATGASQGLLDRKEVLGGVIAVGLVGLIFAVCLVGFMLYRMKKKDEGSYSLEEPKQANGGAYQKPTKQEEFYA Predict reactive species |
| Full Name | syndecan 1 |
| Calculated Molecular Weight | 32.5 kDa |
| GenBank Accession Number | BC008765 |
| Gene Symbol | CD138 |
| Gene ID (NCBI) | 6382 |
| RRID | AB_2882452 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P18827 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Syndecan-1 (SDC1), also named CD138, is an integral membrane protein. It participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a heparan sulfate proteoglycan expressed on the surface of, and actively shed by, myeloma cells. Altered syndecan-1 expression has been detected in several different tumor types. SDC1 was regarded as a useful marker for labeling normal and neoplastic plasma cells and plasmacytoid lymphomas.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CD138/Syndecan-1 antibody 67155-1-Ig | Download protocol |
| IHC protocol for CD138/Syndecan-1 antibody 67155-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CD138/Syndecan-1 Monoclonal antibody (4F3A8), catalog number 67155-1-Ig, has 7 total citations. These appear in journals including Autophagy, Nature Communications, Advanced Science, Developmental Cell, Experimental Cell Research, Placenta, and Translational Cancer Research. The associated research fields span glioblastoma radiosensitivity and autophagy, pan-cancer biomarker detection, multiple myeloma immunity, trophoblast development, glomerulonephritis, preeclampsia-related ferroptosis, and lung adenocarcinoma prognosis.
| Species | Application | Title |
|---|---|---|
Autophagy SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes | ||
Nat Commun Establishment and validation of a plasma oncofetal chondroitin sulfated proteoglycan for pan-cancer detection | ||
Adv Sci (Weinh) Targeting of the CD161 Inhibitory Receptor Enhances Bone-Marrow-Resident Memory CD8(+) T-Cell-Mediated Immunity against Multiple Myeloma. | ||
Dev Cell SP6 controls human cytotrophoblast fate decisions and trophoblast stem cell establishment by targeting MSX2 regulatory elements | ||
Exp Cell Res ZFP36 and CEBPA are macrophage-associated prognostic biomarkers linked to glomerular endothelial inflammation in ANCA-associated glomerulonephritis. | ||
Placenta HIF-2α / HILPDA promotes ferroptosis sensitivity in placenta trophoblast cells of early-onset preeclampsia. |
