Tested Applications
| Positive WB detected in | HeLa cells, MCF-7 cells, rat liver tissue, mouse brain tissue, rat brain tissue, MDA-MB-231 cells |
| Positive IHC detected in | human breast cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14776-1-AP targets SEC22B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6077 Product name: Recombinant human SEC22B protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 2-215 aa of BC001364 Sequence: VLLTMIARVADGLPLAASMQEDEQSGRDLQQYQSQAKQLFRKLNEQSPTRCTLEAGAMTFHYIIEQGVCYLVLCEAAFPKKLAFAYLEDLHSEFDEQHGKKVPTVSRPYSFIEFDTFIQKTKKLYIDSRARRNLGSINTELQDVQRIMVANIEEVLQRGEALSALDSKANNLSSLSKKYRQDAKYLNMRSTYAKLAAVAVFFIMLIVYVRFWWL Predict reactive species |
| Full Name | SEC22 vesicle trafficking protein homolog B (S. cerevisiae) |
| Calculated Molecular Weight | 25 kDa |
| Observed Molecular Weight | 24 kDa |
| GenBank Accession Number | BC001364 |
| Gene Symbol | SEC22B |
| Gene ID (NCBI) | 9554 |
| RRID | AB_2186083 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75396 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein receptor (SNARE) proteins are critical components of the membrane fusion machinery (PMID: 20163564). SEC22B is a SNARE involved in endoplasmic reticulum (ER)/Golgi membrane trafficking. SEC22B localizes to the ER-Golgi intermediate compartment (ERGIC) and has been identified as a regulator of antigen crosspresentation (PMID: 22153078).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SEC22B antibody 14776-1-AP | Download protocol |
| IHC protocol for SEC22B antibody 14776-1-AP | Download protocol |
| WB protocol for SEC22B antibody 14776-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SEC22B Polyclonal antibody (Cat# 14776-1-AP) has 8 total citations published in journals including Cell Metabolism, Nature Cell Biology, Autophagy, Nature Communications, Science Advances, Cell Death & Disease, Brain Research Bulletin, and Journal of Neuroscience. Research fields span lipid homeostasis, ER/Golgi membrane trafficking, secretory autophagy, mitophagy, neuroprotection in traumatic brain injury, neuronal ischemia-reperfusion injury, glioblastoma chemoresistance, and metastatic progression in triple-negative breast cancer.
| Species | Application | Title |
|---|---|---|
Cell Metab Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans. | ||
Nat Cell Biol The SNARE Sec22b has a non-fusogenic function in plasma membrane expansion. | ||
Autophagy Secretory autophagy mediates SLC16A3/MCT4-dependent lactate secretion to drive metastatic progression in triple-negative breast cancer. | ||
Nat Commun Autophagy-independent role of ATG9A vesicles as carriers for galectin-9 secretion
| ||
Sci Adv Integrated proteogenetic analysis reveals the landscape of a mitochondrial-autophagosome synapse during PARK2-dependent mitophagy. | ||
Cell Death Dis Autophagy-dependent secretion of ENO1 mediates chemoresistance of glioblastoma and tumor microenvironment remodeling. |
